{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/86704"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/86704","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Integration and Excision Mechanism of Bacteroides Conjugative Transposons","abstract":"The third project is about the regulatory mechanism of RteB on CTnDOT. RteA and RteB are shown to be global regulators that regulate many genes on Bacteroides host. Whether RteB regulates CTnDOT and NBU1 excision directly or not is investigated in this study. Both RteB and the mutant are over-expressed in E. coli and partially purified. RteB is a soluble protein and gel shift result shows that RteB binds to 168bp upstream of rteC promoter region. However, the mutant did not bind to any probes tested. Footprint assay is required to determine the exact binding site on rteC promoter region.","abstract_html":"The third project is about the regulatory mechanism of RteB on CTnDOT. RteA and RteB are shown to be global regulators that regulate many genes on Bacteroides host. Whether RteB regulates CTnDOT and NBU1 excision directly or not is investigated in this study. Both RteB and the mutant are over-expressed in E. coli and partially purified. RteB is a soluble protein and gel shift result shows that RteB binds to 168bp upstream of rteC promoter region. However, the mutant did not bind to any probes tested. Footprint assay is required to determine the exact binding site on rteC promoter region.","abstract_has_math":false,"creators":["Song, Bo"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":["Abigail Salyers"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-28T15:17:31Z","date_published":"2015-09-28T15:17:31Z","updated_at":"2026-07-22T22:26:27Z","subjects":["Biology, Microbiology"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3314901"],"render_values":[{"text":"(MiAaPQ)AAI3314901","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/86704","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Abigail Salyers"]},{"key":"dc:creator","label":"Author","values":["Song, Bo"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-28T15:17:31Z","10000-01-01","2008"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Microbiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/86704","(MiAaPQ)AAI3314901"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The third project is about the regulatory mechanism of RteB on CTnDOT. 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Whether RteB regulates CTnDOT and NBU1 excision directly or not is investigated in this study. Both RteB and the mutant are over-expressed in E. coli and partially purified. RteB is a soluble protein and gel shift result shows that RteB binds to 168bp upstream of rteC promoter region. However, the mutant did not bind to any probes tested. Footprint assay is required to determine the exact binding site on rteC promoter region.","Made available in DSpace on 2015-09-28T15:17:31Z (GMT). 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