The Integration and Excision Mechanisms of the Bacteroides Conjugative Transposon CTnDOT

A critical requirement for studying transposition is the availability of a defined in vitro transposition system. Using the partial-purified CTnDOT Int protein, an in vitro integration system was developed and a host factor(s) from Bacteroides cell extract was shown to be important for integration. The CTnDOT Int protein catalyzes the in vitro integration reactions by a coupling sequence mechanism similar to the in vivo reaction. Purified E. coli integration host factor (IHF) can substitute for the Bacteroides host factor(s) and significantly stimulates in vitro integration.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Cheng, Qi

Contributors dc:contributor

• Gardner, Jeffrey F.

• Biology, Genetics

Language dc:language

eng

Identifier

(MiAaPQ)AAI3044068

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/86636