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Purified E. coli integration host factor (IHF) can substitute for the Bacteroides host factor(s) and significantly stimulates in vitro integration.","abstract_html":"A critical requirement for studying transposition is the availability of a defined in vitro transposition system. Using the partial-purified CTnDOT Int protein, an in vitro integration system was developed and a host factor(s) from Bacteroides cell extract was shown to be important for integration. The CTnDOT Int protein catalyzes the in vitro integration reactions by a coupling sequence mechanism similar to the in vivo reaction. Purified E. coli integration host factor (IHF) can substitute for the Bacteroides host factor(s) and significantly stimulates in vitro integration.","abstract_has_math":false,"creators":["Cheng, Qi"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":["Gardner, Jeffrey F."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-28T15:17:05Z","date_published":"2015-09-28T15:17:05Z","updated_at":"2026-07-22T22:26:27Z","subjects":["Biology, Genetics"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3044068"],"render_values":[{"text":"(MiAaPQ)AAI3044068","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/86636","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Gardner, Jeffrey F."]},{"key":"dc:creator","label":"Author","values":["Cheng, Qi"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-28T15:17:05Z","10000-01-01","2002"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Genetics"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/86636","(MiAaPQ)AAI3044068"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["A critical requirement for studying transposition is the availability of a defined in vitro transposition system. 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