Molecular and Genetic Analysis of the Role of Ku in Silencing and Telomere Function in Saccharomyces Cerevisiae

The binding of Ku at the telomere is an integral part of maintaining the specialized chromatin structure required for genomic stability at the telomeres. To gain further insight into the signals and complexes involved in the multiple processes contributing to normal telomere function, we performed a Synthetic Genetic Array analysis focusing on genes with roles in silencing (YKU70, SIR1, SAS2, SAS5, SET1, ASF1) and telomere length regulation (YKU70, BDF1, ANC1, RAD50, TEL1, RAD52). Double mutant analysis revealed functional relationships between genes by generating a network of 241 synthetic interactions between 144 genes at 30°C and a network of 355 synthetic lethal interactions between 146 genes at 37°C. The genetic interactions highlighted the underappreciated role of chromatin remodeling proteins in normal telomere function. Additionally, a screen for suppressors of Ku senescence at 37°C identified a role for inositol phosphate signaling in telomere length regulation.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Cell and Developmental Biology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Vandre, Catherine

Contributors dc:contributor

• Belmont, Andrew S.

• Biology, Molecular

Language dc:language

eng

Identifier

(MiAaPQ)AAI3347551

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/86323