Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"Synthetic lethal interactions"”.
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Identification of novel synthetic lethal interactions using multiplexed CRISPR-Cas9 screening
… The aim of this project is to identify novel synthetic lethal interactions within melanoma. Synthetic lethality describes a relationship between two genes, where loss of either of the pair is compatible with cellular viability, but simultaneous loss of both genes induces cell death. This …
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Genome-wide CRISPR Screening in FBXW7-mutant Cells Uncovers Multiple Druggable Synthetic Lethal Interactions Involving Cell Cycle Control
… several isogenic cell lines to identify genetic interactions that are synthetic lethal with common cancer-associated mutations in the Wnt signalling pathway and that could therefore represent new therapeutic targets. From this work we identified two novel druggable synthetic lethal interactions …
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The IN4MER CRISPR/Cas12a Multiplex Knockout Platform and Its Applications
<p>Discovering synthetic lethal interactions between genes holds the key to uncovering cancer vulnerabilities, enabling the development of more effective drugs for patients. However, identifying these vulnerabilities in the complex genome of human, which comprises thousands of genes, poses a …
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Expanding cancer therapy options through genome-scale identification of synthetic lethal paralogs
Synthetic lethal therapies have the potential to expand cancer treatment options. Synthetic lethality is a form of context-dependent gene essentiality in which dual inactivation of a gene pair leads to cell death but single-gene inactivation does not affect viability. When one synthetic lethal gene …
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Application of CRISPR/Cas9 screening to study cancer drivers and to identify novel cancer vulnerabilities
… to treat these cancers is the exploitation of synthetic lethal interactions. Synthetic lethality describes an interaction between two genes, where loss of one gene alone does not affect viability but loss of both genes induces cell death. Inhibiting the synthetic lethal partner of a tumour …
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Exploiting Chemogenetic and Genetic Interactions In Human Cells As An Avenue For New Therapeutic Opportunities
… which enables the identification of chemogenetic interactions, or gene mutations that enhance or suppress the activity of a drug, and 2) combinatorial editing, which facilitates the examination of possible genetic interaction between the two perturbed genes. Both chemogenetic and genetic …
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Molecular and Genetic Analysis of the Role of Ku in Silencing and Telomere Function in Saccharomyces Cerevisiae
… to normal telomere function, we performed a Synthetic Genetic Array analysis focusing on genes with roles in silencing (YKU70, SIR1, SAS2, SAS5, SET1, ASF1) and telomere length regulation (YKU70, BDF1, ANC1, RAD50, TEL1, RAD52). Double mutant analysis revealed functional relationships between …
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Rational drug combinations to selectively target 9p21-deleted bladder cancer
… therapies. In this project we investigated the synthetic lethal vulnerabilities exposed by 9p21 loss in BLCA, the most common copy-number alteration in this tumor. Previous studies have investigated 9p21 loss across several cancer types and identified PRMT5 and MAT2A as synthetic lethal …
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Biases and Blind-Spots In Genome-Wide Crispr-Cas9 Knockout Screens
… screening technology and discuss the paralog synthetic lethal interactions that we have identified, which have escaped detection in monogenic CRISPR-Cas9 knockout screens. Collectively, these observations reveal significant biases and blind-spots in the analysis of CRISPR-based functional …