{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/86323"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/86323","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Molecular and Genetic Analysis of the Role of Ku in Silencing and Telomere Function in Saccharomyces Cerevisiae","abstract":"The binding of Ku at the telomere is an integral part of maintaining the specialized chromatin structure required for genomic stability at the telomeres. To gain further insight into the signals and complexes involved in the multiple processes contributing to normal telomere function, we performed a Synthetic Genetic Array analysis focusing on genes with roles in silencing (YKU70, SIR1, SAS2, SAS5, SET1, ASF1) and telomere length regulation (YKU70, BDF1, ANC1, RAD50, TEL1, RAD52). Double mutant analysis revealed functional relationships between genes by generating a network of 241 synthetic interactions between 144 genes at 30°C and a network of 355 synthetic lethal interactions between 146 genes at 37°C. The genetic interactions highlighted the underappreciated role of chromatin remodeling proteins in normal telomere function. Additionally, a screen for suppressors of Ku senescence at 37°C identified a role for inositol phosphate signaling in telomere length regulation.","abstract_html":"The binding of Ku at the telomere is an integral part of maintaining the specialized chromatin structure required for genomic stability at the telomeres. To gain further insight into the signals and complexes involved in the multiple processes contributing to normal telomere function, we performed a Synthetic Genetic Array analysis focusing on genes with roles in silencing (YKU70, SIR1, SAS2, SAS5, SET1, ASF1) and telomere length regulation (YKU70, BDF1, ANC1, RAD50, TEL1, RAD52). Double mutant analysis revealed functional relationships between genes by generating a network of 241 synthetic interactions between 144 genes at 30°C and a network of 355 synthetic lethal interactions between 146 genes at 37°C. The genetic interactions highlighted the underappreciated role of chromatin remodeling proteins in normal telomere function. Additionally, a screen for suppressors of Ku senescence at 37°C identified a role for inositol phosphate signaling in telomere length regulation.","abstract_has_math":false,"creators":["Vandre, Catherine"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Cell and Developmental Biology","degree_department":null,"school":null,"contributors":["Belmont, Andrew S."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-28T15:03:21Z","date_published":"2015-09-28T15:03:21Z","updated_at":"2026-07-22T22:26:27Z","subjects":["Biology, Molecular"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3347551"],"render_values":[{"text":"(MiAaPQ)AAI3347551","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/86323","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Belmont, Andrew S."]},{"key":"dc:creator","label":"Author","values":["Vandre, Catherine"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-28T15:03:21Z","10000-01-01","2008"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Cell and Developmental Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Molecular"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/86323","(MiAaPQ)AAI3347551"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The binding of Ku at the telomere is an integral part of maintaining the specialized chromatin structure required for genomic stability at the telomeres. To gain further insight into the signals and complexes involved in the multiple processes contributing to normal telomere function, we performed a Synthetic Genetic Array analysis focusing on genes with roles in silencing (YKU70, SIR1, SAS2, SAS5, SET1, ASF1) and telomere length regulation (YKU70, BDF1, ANC1, RAD50, TEL1, RAD52). Double mutant analysis revealed functional relationships between genes by generating a network of 241 synthetic interactions between 144 genes at 30°C and a network of 355 synthetic lethal interactions between 146 genes at 37°C. The genetic interactions highlighted the underappreciated role of chromatin remodeling proteins in normal telomere function. Additionally, a screen for suppressors of Ku senescence at 37°C identified a role for inositol phosphate signaling in telomere length regulation.","Made available in DSpace on 2015-09-28T15:03:21Z (GMT). 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To gain further insight into the signals and complexes involved in the multiple processes contributing to normal telomere function, we performed a Synthetic Genetic Array analysis focusing on genes with roles in silencing (YKU70, SIR1, SAS2, SAS5, SET1, ASF1) and telomere length regulation (YKU70, BDF1, ANC1, RAD50, TEL1, RAD52). Double mutant analysis revealed functional relationships between genes by generating a network of 241 synthetic interactions between 144 genes at 30°C and a network of 355 synthetic lethal interactions between 146 genes at 37°C. The genetic interactions highlighted the underappreciated role of chromatin remodeling proteins in normal telomere function. Additionally, a screen for suppressors of Ku senescence at 37°C identified a role for inositol phosphate signaling in telomere length regulation.","Made available in DSpace on 2015-09-28T15:03:21Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3347551.pdf: 13126260 bytes, checksum: ce65f34249c05f7666043a9f38726a87 (MD5) Previous issue date: 2008","Embargo set by: Seth Robbins for item 87604 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","237 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2008."],"dc:identifier":["http://hdl.handle.net/2142/86323","(MiAaPQ)AAI3347551"],"dc:language":["eng"],"dc:subject":["Biology, Molecular"],"dc:title":["Molecular and Genetic Analysis of the Role of Ku in Silencing and Telomere Function in Saccharomyces Cerevisiae"],"dc:type":["text"],"thesis:degree_discipline":["Cell and Developmental Biology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:27Z"}