Transformation of Somatic Embryos of Soybean With Chitinase and Beta-1,3-Glucanase Genes via Particle Bombardment

Chitinase, maize and bean chitinase and maize β-1,3-glucanase gene were transformed into somatic embryos of soybean using the conditions described above. Enzyme activity assays indicated that the transformed progeny had higher activity than that of the wild type and homozygous progeny had higher activity than heterozygous progeny. Northern analysis indicated that the transgenic progeny of line 26 and 29 produced the 1.1 kb mRNA for bean chitinase. The progeny from line 29 also produced an mRNA which was larger than normal size. Pathogenic assays suggested that expression of the transgene could enhance disease resistance to brown stem rot, sudden death syndrome and brown leaf spot at the early stage of plant and disease development.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Crop Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Chanprame, Sontichai

Contributors dc:contributor

• Widholm, Jack M.

• Biology, Genetics

Language dc:language

eng

Identifier

(MiAaPQ)AAI9834661

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/85053