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Showing 1 to 20 of 76 for “"Chitinase"”.
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Experimental and computational studies of a fungal chitinase
Submitted in fulfillment of the requirements of the degree of Doctor of Philosophy: Chemistry, Durban University of Technology, 2015.
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Characterization of Chitinase Activity and Gene Expression in Muskmelon Seeds
Chitinase has been suggested to play a role in defense mechanisms. In this study, the activity and expression of chitinase in muskmelon seeds were investigated. Multiple chitinase isoforms were detected in muskmelon seeds from early development through radicle emergence. One acidic and three basic …
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Role of Chitinase in the development of work-related symptoms in bakery workers
… 포함되어 있다. 따라서 이러한 Chitin 성분 흡입이 되면 면역세포로부터 Chitinase 생성이 증가하면서 유도되는 기도 염증 반응이 제빵공 천식의 병인 기전에 관여하리라 추정하고 있지만, 아직 그 기전은 알려져 있지 않다. 본 연구에서는 제빵공에서 밀가루 분진 노출후 Chitinase 생성 증가가 작업 관련 증상 발생에 관여할 가능성을 탐색하였다. 성남의 한 제빵공장 직원 381명을 대상으로 하였고, 이들은 분진에 노출되지 않은 건강 대조군 106명과 비교하였다. 제빵공의 작업 관련 증상 유무를 판정하기 위해 …
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Generation of Transgenic <i>Medicago Sativa</i> Overexpressing "<i>Osmotin-Chitinase</i>" Gene Chimera
… was developed for over-expressing an <i>“Osmotin-Chitinase</i>” gene chimera in transgenic <i>Medicago</i> that could potentially confer resistance to different biotic and abiotic stresses. Seed germination of several cultivars of <i>Medicago</i> (<i>M. sativa ssp. sativa, M. sativa ssp. falcata, …
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Investigating the key components of the chitinase secretion system in <i>Serratia marcescens</i>
… and these are essential for secretion of chitinases. Using X-ray crystallography, the structure of full-length ChiX was resolved to 1.34 Å, revealing structural similarities to the lysostaphin-type (LAS) family of peptidases. Purified ChiX was shown to possess L-Ala D-Glu endopeptidase …
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Construction des enzymes hybrides entre la chitinase de l'orge et la chitosanase de Streptomyces SP. N174
… la chitosanase de Streptomyces sp. N174 et la chitinase de l'orge. Ces deux protéines comptent parmi les enzymes les mieux étudiées et caractérisées au sein de leurs familles respectives. Toutes les données concernant leurs séquences d'ADN, leurs séquences primaires, leurs structures tertiaires …
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Transformation of Somatic Embryos of Soybean With Chitinase and Beta-1,3-Glucanase Genes via Particle Bombardment
Chitinase, maize and bean chitinase and maize $\beta$-1,3-glucanase gene were transformed into somatic embryos of soybean using the conditions described above. Enzyme activity assays indicated that the transformed progeny had higher activity than that of the wild type and homozygous progeny had …
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PCR-based cloning, characterization, and stress-induced expression of chitinase genes in Kentucky bluegrass (Poa pratensis L.)
Chitinase is an enzyme that catalyzes the hydrolysis of chitin, an essential component of the cell walls of many fungi. Plant chitinases have been implicated in plant defense against pathogens. In plants, chitinase often exists as isoforms and three classes of chitinases have been proposed. In this …
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Isolation and characterization of a chitinase-secreting mutant of mortierella pusilla with altered interaction with the mycoparasite piptocephalis virginiana
… was compared to the wild type strain for their chitinase production in sUbmerged culture. The chitinase isozymes of both wild type and MUT23 were shown by immunoblotting. Eight distinct chitinase molecules were detected. MUT23 showed markedly higher chitinase activity than the wild type cultured …
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Regulation of Glucanohydrolases in Nicotiana Tabacum on the Messenger-Rnalevel (beta-1,3-Glucanase, Chitinase, Cytokinin, Auxin, Complementary-Dna Clone)
… of the abundant enzymes (beta)-1,3-glucanase and chitinase in cultured tobacco (Nicotiana tabacum L. cv. Havana 425) tissues is inhibited by the hormones auxin and cytokinin. As judged by immunosorption and partial proteolysis of the in vitro translation products, poly(A)+ RNA from tobacco tissues …
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Immuno-gluorescence study of five zygomycetous fungi with two chitin-binding probes
… was prepared against a purified microsomal chitinase isolated from the fungus Choanephora cucurbitarum. Indirect immunofluorescence was used to localize chitinase at various developmental stages of five zygomycetous fungi and during abiotrophic mycoparasite interaction with a susceptible and …
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Isolation and Characterization of Chitinases From Dasypus Novemcinctus, the Nine-Banded Armadillo
… was investigated. Both indigenous and endogenous chitinases were studied. Armadillos were collected and samples of their gastric and pancreatic tissues were removed. Tissues were lyophilized and stored at -20° C until they could be resuspended in the appropriate buffers. The extract from …
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Studies on expression of an antifungal gene maize chitinase in tobacco and potato and its effect on growth and the fungal pathogen Rhizoctonia solani
Restriction data tranferred 2014-07-01T11:16:52-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission
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Pengaruh enzim kitinase kasar dari bakteri Pseudomonas pseudomallei dan Klebsiella ozaenae terhadap pertumbuhan, morfologi, dan kadar N-asetilglukosamin Fusarium oxysporum
… was conducted to determine the effect of crude chitinase enzyme isolated from Pseudomonas pseudomallei bacterium, Klebsiella ozaenae, and a combination of both on growth, morphology, and levels of N-acetylglucosamine F.oxysporum. This study was an experimental study using a completely randomized …
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Secretion and regulation of the chitinolytic machinery in <i>Serratia marcescens</i>
… To achieve this S. marcescens secretes three chitinases (ChiA, ChiB and ChiC) as well as a chitin binding protein (CBP21) that together with the periplasmic chitobiase (Chb) form the chitinolytic machinery. <br/>Research in this group had identified two genes (chiW and chiX) encoding a …
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Turfgrass species composition, resistance mechanisms, and management strategy impacts on brown patch incidence and weed encroachment
… was conducted in tall fescue to quantify chitinase activity in different cultivars, elucidate the impact of mowing height and nitrogen fertility on brown patch and bermudagrass (Cynodon dactylon L.) encroachment, to evaluate seeding mixtures of tall fescue with hybrid bluegrass (Poa …
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Characterization of the chitinolytic system during the mycoparasitic interaction between Trichoderma aggressivum f. aggressivum and different host strains of Agaricus bisporus /
… fungi, is the secretion of lytic enzymes such as chitinases, which actively degrade a host's cell wall. Therefore, the intent of this study was to examine the production of chitinase enzymes during the host-parasite interaction of Agaricus bisporus (commercial mushroom) and Triclzodemza …
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Maize Transformation for Resistance to Aspergillus Flavus
The chitinase18 (ch18) and beta-1,3-glucanaseII (glu) genes driven by globulin1 were delivered into H99 and Pa91 type I calli by particle bombardment. Sixteen different transformation events were obtained from H99 calli. Some were crossed with Pa91 the untransformed control and then self-pollinated …
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