{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/85053"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/85053","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Transformation of Somatic Embryos of Soybean With Chitinase and Beta-1,3-Glucanase Genes via Particle Bombardment","abstract":"Chitinase, maize and bean chitinase and maize $\\beta$-1,3-glucanase gene were transformed into somatic embryos of soybean using the conditions described above. Enzyme activity assays indicated that the transformed progeny had higher activity than that of the wild type and homozygous progeny had higher activity than heterozygous progeny. Northern analysis indicated that the transgenic progeny of line 26 and 29 produced the 1.1 kb mRNA for bean chitinase. The progeny from line 29 also produced an mRNA which was larger than normal size. Pathogenic assays suggested that expression of the transgene could enhance disease resistance to brown stem rot, sudden death syndrome and brown leaf spot at the early stage of plant and disease development.","abstract_html":"Chitinase, maize and bean chitinase and maize <span class=\"etd-inline-math\">&beta;</span>-1,3-glucanase gene were transformed into somatic embryos of soybean using the conditions described above. Enzyme activity assays indicated that the transformed progeny had higher activity than that of the wild type and homozygous progeny had higher activity than heterozygous progeny. Northern analysis indicated that the transgenic progeny of line 26 and 29 produced the 1.1 kb mRNA for bean chitinase. The progeny from line 29 also produced an mRNA which was larger than normal size. Pathogenic assays suggested that expression of the transgene could enhance disease resistance to brown stem rot, sudden death syndrome and brown leaf spot at the early stage of plant and disease development.","abstract_has_math":true,"creators":["Chanprame, Sontichai"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Crop Sciences","degree_department":null,"school":null,"contributors":["Widholm, Jack M."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T22:33:38Z","date_published":"2015-09-25T22:33:38Z","updated_at":"2026-07-22T22:26:24Z","subjects":["Biology, Genetics"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI9834661"],"render_values":[{"text":"(MiAaPQ)AAI9834661","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/85053","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Widholm, Jack M."]},{"key":"dc:creator","label":"Author","values":["Chanprame, Sontichai"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T22:33:38Z","10000-01-01","1998"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Crop Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Genetics"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/85053","(MiAaPQ)AAI9834661"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Chitinase, maize and bean chitinase and maize $\\beta$-1,3-glucanase gene were transformed into somatic embryos of soybean using the conditions described above. Enzyme activity assays indicated that the transformed progeny had higher activity than that of the wild type and homozygous progeny had higher activity than heterozygous progeny. Northern analysis indicated that the transgenic progeny of line 26 and 29 produced the 1.1 kb mRNA for bean chitinase. The progeny from line 29 also produced an mRNA which was larger than normal size. Pathogenic assays suggested that expression of the transgene could enhance disease resistance to brown stem rot, sudden death syndrome and brown leaf spot at the early stage of plant and disease development.","Made available in DSpace on 2015-09-25T22:33:38Z (GMT). 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Enzyme activity assays indicated that the transformed progeny had higher activity than that of the wild type and homozygous progeny had higher activity than heterozygous progeny. Northern analysis indicated that the transgenic progeny of line 26 and 29 produced the 1.1 kb mRNA for bean chitinase. The progeny from line 29 also produced an mRNA which was larger than normal size. Pathogenic assays suggested that expression of the transgene could enhance disease resistance to brown stem rot, sudden death syndrome and brown leaf spot at the early stage of plant and disease development.","Made available in DSpace on 2015-09-25T22:33:38Z (GMT). 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