Glyphosate Resistance in Plant Cell Cultures

In the second study, five carrot DHL lines were studied in order to identify the mechanism underlying glyphosate resistance. Uptake experiments, using radiolabelled glyphosate, showed higher or similar absorption levels in the resistant lines compared to the unselected control. Such results exclude uptake as a mechanism of glyphosate resistance. Inhibition studies of EPSPS by glyphosate however showed higher Ki values in three lines. One of these three lines (DHL 25), did not show consistent Ki values, but the observed values varied depending upon the growth stage of the cells at the time of each measurement, suggesting the presence of a less sensitive EPSPS enzyme that fluctuates in amount over time in this line.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Crop Sciences

Grantor

University of Illinois at Urbana-Champaign

Date dc:date

10000-01-01

Author dc:creator

• Papanikou, Efstratia

Contributors dc:contributor

• Widholm, Jack M.

• Biology, Plant Physiology

Language dc:language

eng

Identifier

(MiAaPQ)AAI3101942

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/85012