{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/85012"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/85012","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Glyphosate Resistance in Plant Cell Cultures","abstract":"In the second study, five carrot DHL lines were studied in order to identify the mechanism underlying glyphosate resistance. Uptake experiments, using radiolabelled glyphosate, showed higher or similar absorption levels in the resistant lines compared to the unselected control. Such results exclude uptake as a mechanism of glyphosate resistance. Inhibition studies of EPSPS by glyphosate however showed higher Ki values in three lines. One of these three lines (DHL 25), did not show consistent Ki values, but the observed values varied depending upon the growth stage of the cells at the time of each measurement, suggesting the presence of a less sensitive EPSPS enzyme that fluctuates in amount over time in this line.","abstract_html":"In the second study, five carrot DHL lines were studied in order to identify the mechanism underlying glyphosate resistance. Uptake experiments, using radiolabelled glyphosate, showed higher or similar absorption levels in the resistant lines compared to the unselected control. Such results exclude uptake as a mechanism of glyphosate resistance. Inhibition studies of EPSPS by glyphosate however showed higher Ki values in three lines. One of these three lines (DHL 25), did not show consistent Ki values, but the observed values varied depending upon the growth stage of the cells at the time of each measurement, suggesting the presence of a less sensitive EPSPS enzyme that fluctuates in amount over time in this line.","abstract_has_math":false,"creators":["Papanikou, Efstratia"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Crop Sciences","degree_department":null,"school":null,"contributors":["Widholm, Jack M."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":null,"date_issued":"10000-01-01","date_published":"10000-01-01","updated_at":"2026-07-22T22:26:24Z","subjects":["Biology, Plant Physiology"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3101942"],"render_values":[{"text":"(MiAaPQ)AAI3101942","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/85012","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Widholm, Jack M."]},{"key":"dc:creator","label":"Author","values":["Papanikou, Efstratia"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["10000-01-01","2003","2015-09-25T22:33:26Z"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Crop Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Plant Physiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/85012","(MiAaPQ)AAI3101942"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["In the second study, five carrot DHL lines were studied in order to identify the mechanism underlying glyphosate resistance. Uptake experiments, using radiolabelled glyphosate, showed higher or similar absorption levels in the resistant lines compared to the unselected control. Such results exclude uptake as a mechanism of glyphosate resistance. Inhibition studies of EPSPS by glyphosate however showed higher Ki values in three lines. One of these three lines (DHL 25), did not show consistent Ki values, but the observed values varied depending upon the growth stage of the cells at the time of each measurement, suggesting the presence of a less sensitive EPSPS enzyme that fluctuates in amount over time in this line.","Made available in DSpace on 2015-09-25T22:33:26Z (GMT). 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Uptake experiments, using radiolabelled glyphosate, showed higher or similar absorption levels in the resistant lines compared to the unselected control. Such results exclude uptake as a mechanism of glyphosate resistance. Inhibition studies of EPSPS by glyphosate however showed higher Ki values in three lines. One of these three lines (DHL 25), did not show consistent Ki values, but the observed values varied depending upon the growth stage of the cells at the time of each measurement, suggesting the presence of a less sensitive EPSPS enzyme that fluctuates in amount over time in this line.","Made available in DSpace on 2015-09-25T22:33:26Z (GMT). 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