Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"EPSPS"”.
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EPSPS gene duplication in Palmer amaranth: relative fitness, inheritance, and duplication mechanism of the glyphosate resistance trait
… glyphosate, 5-enolpyruvylshikimate-3-phosphate (EPSPS) is duplicated in the genome 100+ times, resulting in an overproduction of the EPSPS protein. With so much EPSPS enzyme available in each cell, glyphosate only inhibits a fraction of the proteins, leaving the rest to function as normal and …
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Investigating the heritability of resistance fueling copy number variation in glyphosate resistant Amaranthus palmeri
… populations. In Amaranthus palmeri, CNV of the EPSPS gene, the target of glyphosate herbicide, ranges from 1 to over 160 copies, and is the primary genetic determinant of glyphosate resistance. The amplified copies of EPSPS are found as extrachromosomal circular DNA, which raises questions about …
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Examination of target-site-based mechanisms of glyphosate resistance in waterhemp (Amaranthus tuberculatus)
… resistance have been linked with GR waterhemp: EPSPS gene amplification, EPSPS target-site mutations, and reduced translocation. The objective of this research was to investigate the target-site-based mechanisms (EPSPS gene amplification and EPSPS mutations) of glyphosate resistance. Waterhemp …
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Regional whole plant and molecular response of Kochia scoparia to glyphosate
… for glyphosate resistance. Sequence of the EPSPS binding site proline confirmed that amino acid substitution at that residue was not responsible for resistance in K. scoparia. However, quantitative PCR estimates of EPSPS copy number revealed increased copy number in all glyphosate-resistant …
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Structure of the amplified 5-enolpyruvylshikimate-3-phosphate synthase gene in glyphosate-resistant carrot cells and attempts to isolate the anthranilate synthase gene from plants
… 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) DNA of carrot suspension cultured cell lines selected for glyphosate resistance was carried out to determine the mechanism of gene amplification in this plant system. Cloning of the EPSPS gene and 5$\sp\prime$ flanking sequences was carried …
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Glyphosate Resistance in Plant Cell Cultures
… of glyphosate resistance. Inhibition studies of EPSPS by glyphosate however showed higher Ki values in three lines. One of these three lines (DHL 25), did not show consistent Ki values, but the observed values varied depending upon the growth stage of the cells at the time of each measurement, …
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Angiotensin II modulates glutamate-induced depolarizations and synaptic transmission in rat locus coeruleus neurons in vitro.
… to show that excitatory postsynaptic potentials (EPSPs) evoked by electrical stimulation were blocked by Glu receptor antagonists, indicating involvement of EAAs in the mediation of the EPSPs. Sar$\sp1$-AII or AII applied together with peptidase inhibitors depressed the amplitudes of the EPSPs in …
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Frog Spinal Motoneurons: Their Supraspinal Synaptic Input and Anatomical Localization
… the calamus scriptorius were found to produce EPSPs in flexor and extensor motoneurons innervating the hindlimb. These EPSPs appeared to be generated monosynaptically on the somas and proximal dendrites of the motoneurons. Chromatolysis was used to map the location of motoneurons innervating …
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Studies on preconditioning with adenosine, glutamate and ouabain in rat hippocampal slices
… paradigms upon the extracellularly evoked field epsps, orthodromic and antidromic population spikes. Western blotting was used to detect any changes in the levels of HSP72 in the slices that may have occurred as a result of the depolarizing agents or the preconditioning treatments. It was first …
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From the simple to the complex: an examination of different herbicide resistances
… 5-enolypyruvyl-shikimate-3-phosphate synthase (EPSPS) gene]. Sequencing of the entire TennGR goosegrass EPSPS gene was performed and compared to that of the sensitive Tennessee population (TennGS). The results indicated that the population did contain the anticipated Pro106Ser mutation. An F2 …
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Entwicklung einer Hydrophobin-basierten funktionalisierten Oberfläche für den optischen Nachweis von Glyphosat
… der 5-Enolpyruvylshikimat-3-phosphatsynthase (EPSPS, hier aus dem Bakterium Escherichia coli (EcEPSPS)) und dem zur Selbstassemblierung an hydrophilen/hydrophoben Grenzflächen befähigten Hydrophobin Ccg2 aus Neurospora crassa erzeugt, welche für die Oberflächenfunktionalisierung eingesetzt …
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Calcium dynamics in dendrites and spines of spiny neurons in the somatosensory ‘barrel’ cortex of the rat
… evoked excitatory postsynaptic potentials (EPSPs) resulted in a synapse specific, local increase in Ca2+. Pairing both stimuli at different inter-stimulus intervals revealed a precisely tuned coincidence detection mechanism for pre- and postsynaptic activity, coded in the peak Ca2+ transient …
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Functional implications of behavioral experience effects upon the rat visual cortex
… sensitive NMDA glutamate receptor-mediated EPSPs (NMDA EPSPs), which were involved in cortical LTP production, were significantly larger in the EC rat visual cortex than in the IC. This indicated that cellular mechanisms that mediate electrical LTP may also underlie ""behavioral LTP"". Thus, …
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Effect of Background Synaptic Activity on Excitatory-Postsynaptic Potential-Spike Coupling
… vivo</em>, which may impact the coupling between EPSPs and spikes. We mimicked the<em> in vivo</em> synaptic activity of the cell with the dynamic clamp system. We recorded from pyramidal cells in neocortical slices<em> in vitro</em> to investigate how timing and probability of spike generation in …
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Discovery, surveillance, and management of herbicide-resistant amaranthus spp
… as 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) and protoporphyrinogen oxidase (PPO), have become widespread within a narrow subset of the Amaranthus genus, such as A. tuberculatus and A. palmeri. Species within the genus possesses a distinct geographic distribution. Maintenance of these …
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Effect of Temperature and Chemical Additives on the Efficacy of the Herbicides Glufosinate and Glyphosate in Weed Management of Liberty-Link and Roundup-Ready Soybeans
… of 5-enolpyruvylshikimate- 3-phosphate synthase (EPSPS), which confers glyphosate resistance. Field and greenhouse studies were conducted to determine the efficacy of glufosinate and glyphosate on annual and perennial weeds. Also to determine whether the use of ammonium sulfate (AMS) or pelargonic …
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Long Term Plasticity Induced by Intracellular Tetanization in the Rat Auditory Cortex
… evoked excitatory postsynaptic potentials (EPSPs) in layer 2/3 pyramidal cells in slices from rat auditory cortex. Two types of inputs were observed. Regular inputs (observed at 68 of 90 inputs, 76%) were characterized by a high-variance, gap-free distribution of response amplitudes, while …
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The Mechanisms And Roles Of Feedback Loops For Visual Processing
… activity characterized with a barrage of EPSPs and occasional spikes. Further experiments reveal the involvement of GABA in mediating the spontaneous synaptic inputs to the Ipc neurons. Next we investigate the mechanisms of oscillatory bursting in Ipc, which is observed <italic>in …
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Influence of perforant path synaptic excitation on the initiation of hippocampal sharp-wave ripple activity in vitro
… of PP afferents evoked short-latency field EPSPs in CA3 that were often followed by precisely timed evoked SWRs (eSWR). The network and single-cell characteristics of sSWRs and eSWRs were indistinguishable, indicative of a common patter generator. PP stimuli that followed sSWRs too closely …
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Mechanisms of neuronal integration in adrenomedullary sympathetic preganglionic neurons.
… the ipsi- and contralateral funiculi evoked fast EPSPs in all AD-SPN that were mediated by both NMDA and non-NMDA receptors. A subpopulation of AD-SPN received fast IPSPs mediated by GABA acting via GABAA receptors. A train of stimuli (4 x 10Hz) in ipsi- and contralateral funiculi also evoked a …
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