The Estrogen Response Element and the KRAB Repression Domain as Modulators of Estrogen Receptor Action

Repressing expression of estrogen regulated genes would greatly facilitate studies of their function. Therefore we transformed ER into a transcription repressor by fusing the KRAB transcription repressor domain of the Kox1 protein to ER and various ER mutants. Incorporation of DBD mutations conferring increased ERE affinity previously identified in a genetic selection, allowed us to effectively repress in a ligand dependent fashion both basal and estrogen receptor induced transcription from a promoter containing a single non consensus ERE. These ER-KRAB chimeras therefore constitute powerful tools for the study of estrogen regulated gene expression.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• De Haan, Georgius

Contributors dc:contributor

• Shapiro, David J.

• Chemistry, Biochemistry

Language dc:language

eng

Identifier

(MiAaPQ)AAI9953002

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/84908