{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/84908"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/84908","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"The Estrogen Response Element and the KRAB Repression Domain as Modulators of Estrogen Receptor Action","abstract":"Repressing expression of estrogen regulated genes would greatly facilitate studies of their function. Therefore we transformed ER into a transcription repressor by fusing the KRAB transcription repressor domain of the Kox1 protein to ER and various ER mutants. Incorporation of DBD mutations conferring increased ERE affinity previously identified in a genetic selection, allowed us to effectively repress in a ligand dependent fashion both basal and estrogen receptor induced transcription from a promoter containing a single non consensus ERE. These ER-KRAB chimeras therefore constitute powerful tools for the study of estrogen regulated gene expression.","abstract_html":"Repressing expression of estrogen regulated genes would greatly facilitate studies of their function. Therefore we transformed ER into a transcription repressor by fusing the KRAB transcription repressor domain of the Kox1 protein to ER and various ER mutants. Incorporation of DBD mutations conferring increased ERE affinity previously identified in a genetic selection, allowed us to effectively repress in a ligand dependent fashion both basal and estrogen receptor induced transcription from a promoter containing a single non consensus ERE. These ER-KRAB chimeras therefore constitute powerful tools for the study of estrogen regulated gene expression.","abstract_has_math":false,"creators":["De Haan, Georgius"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biochemistry","degree_department":null,"school":null,"contributors":["Shapiro, David J."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T22:28:27Z","date_published":"2015-09-25T22:28:27Z","updated_at":"2026-07-22T22:26:24Z","subjects":["Chemistry, Biochemistry"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI9953002"],"render_values":[{"text":"(MiAaPQ)AAI9953002","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/84908","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Shapiro, David J."]},{"key":"dc:creator","label":"Author","values":["De Haan, Georgius"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T22:28:27Z","10000-01-01","1999"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry, Biochemistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/84908","(MiAaPQ)AAI9953002"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Repressing expression of estrogen regulated genes would greatly facilitate studies of their function. Therefore we transformed ER into a transcription repressor by fusing the KRAB transcription repressor domain of the Kox1 protein to ER and various ER mutants. Incorporation of DBD mutations conferring increased ERE affinity previously identified in a genetic selection, allowed us to effectively repress in a ligand dependent fashion both basal and estrogen receptor induced transcription from a promoter containing a single non consensus ERE. These ER-KRAB chimeras therefore constitute powerful tools for the study of estrogen regulated gene expression.","Made available in DSpace on 2015-09-25T22:28:27Z (GMT). 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Therefore we transformed ER into a transcription repressor by fusing the KRAB transcription repressor domain of the Kox1 protein to ER and various ER mutants. Incorporation of DBD mutations conferring increased ERE affinity previously identified in a genetic selection, allowed us to effectively repress in a ligand dependent fashion both basal and estrogen receptor induced transcription from a promoter containing a single non consensus ERE. These ER-KRAB chimeras therefore constitute powerful tools for the study of estrogen regulated gene expression.","Made available in DSpace on 2015-09-25T22:28:27Z (GMT). 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