The Use of High-Affinity T Cell Receptors to Study Specificity in the 2C T Cell System

Finally, soluble high-affinity TCRs are promising reagents for the detection and diagnosis of specific virus or tumor antigens (peptide-MHC) on the cell surface. In addition, the TCRs would provide a useful probe for tracking the processing and presentation of specific antigens. Chapter 6 discusses the development of soluble single-chain TCRs with enhanced properties, such as increased avidity and signal amplification, to detect antigen. The results show that tetrameric and multi-fluorophore labeled forms of the high-affinity 2C TCR called m67 were capable of detecting low levels of specific peptide-MHC on the surface of both tumor cells and when the antigen was cross-presented on stromal cells in a solid tumor.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Bowerman, Natalie A.

Contributors dc:contributor

• Kranz, David M.

• Health Sciences, Immunology

Language dc:language

eng

Identifier

(MiAaPQ)AAI3391888

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/84864