{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/84864"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/84864","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"The Use of High-Affinity T Cell Receptors to Study Specificity in the 2C T Cell System","abstract":"Finally, soluble high-affinity TCRs are promising reagents for the detection and diagnosis of specific virus or tumor antigens (peptide-MHC) on the cell surface. In addition, the TCRs would provide a useful probe for tracking the processing and presentation of specific antigens. Chapter 6 discusses the development of soluble single-chain TCRs with enhanced properties, such as increased avidity and signal amplification, to detect antigen. The results show that tetrameric and multi-fluorophore labeled forms of the high-affinity 2C TCR called m67 were capable of detecting low levels of specific peptide-MHC on the surface of both tumor cells and when the antigen was cross-presented on stromal cells in a solid tumor.","abstract_html":"Finally, soluble high-affinity TCRs are promising reagents for the detection and diagnosis of specific virus or tumor antigens (peptide-MHC) on the cell surface. In addition, the TCRs would provide a useful probe for tracking the processing and presentation of specific antigens. Chapter 6 discusses the development of soluble single-chain TCRs with enhanced properties, such as increased avidity and signal amplification, to detect antigen. The results show that tetrameric and multi-fluorophore labeled forms of the high-affinity 2C TCR called m67 were capable of detecting low levels of specific peptide-MHC on the surface of both tumor cells and when the antigen was cross-presented on stromal cells in a solid tumor.","abstract_has_math":false,"creators":["Bowerman, Natalie A."],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biochemistry","degree_department":null,"school":null,"contributors":["Kranz, David M."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T22:28:14Z","date_published":"2015-09-25T22:28:14Z","updated_at":"2026-07-22T22:26:24Z","subjects":["Health Sciences, Immunology"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3391888"],"render_values":[{"text":"(MiAaPQ)AAI3391888","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/84864","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Kranz, David M."]},{"key":"dc:creator","label":"Author","values":["Bowerman, Natalie A."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T22:28:14Z","10000-01-01","2009"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Health Sciences, Immunology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/84864","(MiAaPQ)AAI3391888"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Finally, soluble high-affinity TCRs are promising reagents for the detection and diagnosis of specific virus or tumor antigens (peptide-MHC) on the cell surface. 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