Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 60 for “"Signal amplification"”.
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Photopolymerization-based signal amplification : mechanistic characterization and practical implementation
Polymerization-based signal amplification is an approach to biosensing that leverages the amplification inherent to radical polymerization to enhance signal associated with molecular recognition. This versatile technique has been implemented with a number of radical polymerization chemistries, …
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Increased sensitivity for lateral flow immunoassays through signal amplification methods
… a simple and rapid assay procedure, we proposed signal enhancement methods of antigen detection using three approaches: (1) increasing the absorbance of gold nanoparticles using gold enhancement and silver enhancement solutions, (2) enzymatic creation and precipitation of visible dyes, and (3) …
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Super-aptamer bio-imprinted hydrogels : an investigation into the optimization and characterization of cross-linked polymeric materials displaying macromolecular amplified responses
… cannot be achieved directly, but requires signal amplification by catalysts, macromolecules, metal surfaces or supramolecular aggregates. The rapidly progressing field of macromolecular signal amplification has been advanced using conjugated polymers, chirality in polymers, solvating …
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Characterisation of optical fibre amplifiers: amplifiers for the 632.8 nm He-Ne wavelength in praseodymium-doped ZBLAN
An investigation of small-signal amplification on the 3Po-3F2 transition in a praseodymium-doped fluorozirconate optical fibre at the 632.8 nm He-Ne wavelength, has been conducted. The optical gain is fundamentally related to the spectroscopic characteristics of praseodymium-doped in a …
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Probing surface protein patterning in biological systems using fluorescence nanoscopy
… substrates to elucidate key phenomenon such as signal amplification in bacteria during chemotaxis. In B. subtilis, we observed reorganization in arrays of McpB receptor proteins, key chemotactic receptors for asparagine sensing, upon exposure to saturating stimulant concentrations. More …
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Supramolecular assemblies of conjugated sensory polymers and the optimization of transport properties
… to the surface detection layer toward ultimate signal amplification has been discussed. Third, the role of chemical structure of a sensory polymer in the selectivity of a conjugated polymer-based fluorescent sensor has been examined. In two different sensory systems for the detection of …
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Boosting Biodetection Signals via Photopolymerization: Strategies for Photocatalyst Amplification
Photopolymerization-based signal amplification (PBA) is a method to enhance biodetection signals by coupling molecular recognition events with photocatalyst labels (eosin Y) and amplifying through visible light-initiated free radical polymerization. Leveraging inherent amplification in the radical …
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The Use of High-Affinity T Cell Receptors to Study Specificity in the 2C T Cell System
… properties, such as increased avidity and signal amplification, to detect antigen. The results show that tetrameric and multi-fluorophore labeled forms of the high-affinity 2C TCR called m67 were capable of detecting low levels of specific peptide-MHC on the surface of both tumor cells and …
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Advances in Microfluidics and DNA Nanotechnology for Biomolecular Analysis
… mobility, combined with isothermal signal amplification and quantitation by a DNA nanocircuit. Micro-electrophoresis is then applied to the characterisation of oligomeric protein complexes, which are implicated in the pathogenesis of misfolding diseases. In this study, …
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Paper-based Molecular Technologies for Faster, More Accessible Infectious Disease Diagnostics
… detection was performed either via enzymatic amplification or eosin photopolymerization amplification, a visible light-initiated radical polymerization method for colorimetric signal amplification in diagnostic assays. To better understand the eosin photopolymerization reaction mechanism and …
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Development of a concentration-enhanced mobility shift assay platform for aptamer-based biomarker detection and kinase profiling
… which satisfies these criteria, but the lack of amplification method is a bottleneck that limits their use for sensitive applications. To address this issue, this thesis explores physical signal amplification means to increase the sensitivities of homogeneous assays. We identified several key …
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Amplifying signals in the tumor microenvironment for drug development and diagnostics
… (TME) with engineering principles of signal amplification. Tumors are intricately tied to their local and systemic microenvironments for tumor progression, and key to orchestrating such interactions are secreted molecules. Targeting these abundant molecules that are accessible …
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Cable Noise in Impedance Measurements
… it is added prior to the probe electronics signal amplification and conditioning. This thesis describes a study of the electromagnetic attributes of the current prototype cabling design with particular emphasis on the quantitative investigation of its sources of noise. A method for …
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Application of polymerization-based amplification in point-of-care diagnostics
… settings. Photo-initiated polymerization-based amplification (PBA) is a technology that allows detection of a surface-bound analyte through co-localization of a visible-light photoinitiator with the analyte present on the surface. In the presence of an appropriate dose of light and monomers, a …
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Studying DNA-Directed Nanoassembly, Hybridization and Mismatch-Discrimination via Plasmon Scattering of Gold Nanoparticles
… quantification without the need for additional signal amplification.
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Nanomaterials for the detection of cancer-associated biomarkers
… probes demonstrated a 5 to 8-fold fluorescence signal amplification, extended circulation time, and high image contrast in a mouse tumor model. Lastly, differential phage display selection was performed on human prostate cancer cells with low and high metastatic potentials to (1) identify …
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Design and characterization of a low cost dual differential proving ring force sensor utilizing Hall-effect sensors
… negative attributes including: high cost due to signal amplification instrumentation, high temperature sensitivity, and only moderate dynamic range. The goal of the research herein described was to design and test a low cost, high dynamic range force sensor. Hall-Effect sensors have high …
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Engineering Low Noise Organic Electrochemical Transistors for Electrophysiology Applications
… for recording high quality electrophysiology signals is the organic electrochemical transistor (OECT). OECTs have been fabricated onto flexible substrates from biocompatible materials and have been shown to provide higher signal-to-noise ratio (SNR) than electrodes. Their unique properties can …
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DNA Walkers: Emerging Analytical Applications, Biomolecular-Nanomaterial Probes and Biomolecule Sensors
… dynamic interaction, and capabilities for signal amplification, programmable signal transduction, high directionality, and predictable analytical performance on the basis of Watson-Crick base paring rules, this class of dynamic DNA nanodevice has gained great attention from the analytical …
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Hand Pattern Recognition Using Smart Band
… accelerometer senses the hand motions. The EMG signal is passed through analog front-end module for noise filtering and signal amplification. An ARM Cortex processor converts the analog EMG and accelerometer signal into digital and transmits to a PC via Bluetooth protocol. On the receiver …
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