Natural and Engineered Promiscuity Within the Enolase Superfamily

The dehydration of both D-mannonate, via syn-elimination, and D-altronate, via anti-elimination, is analogous to the stereochemical promiscuity engineered into the GlucD active site. The redesign of GlucD and the characterization of RspA illustrate the same type of promiscuity: syn and anti dehydrations in the same active site with different substrates, which results from proton abstraction of stereochemically equivalent protons. These experiments illustrate how Nature could have utilized the (beta/alpha)8-barrel as a scaffold to evolve new activities.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Millikin, Cheri Lynn

Contributors dc:contributor

• Gerlt, John A.

• Chemistry, Biochemistry

Language dc:language

eng

Identifier

(MiAaPQ)AAI3199089

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/84818