{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/84818"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/84818","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Natural and Engineered Promiscuity Within the Enolase Superfamily","abstract":"The dehydration of both D-mannonate, via syn-elimination, and D-altronate, via anti-elimination, is analogous to the stereochemical promiscuity engineered into the GlucD active site. The redesign of GlucD and the characterization of RspA illustrate the same type of promiscuity: syn and anti dehydrations in the same active site with different substrates, which results from proton abstraction of stereochemically equivalent protons. 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