Estrogen Regulation of Proteinase Inhibitor 9 Gene Expression and Interleukin-1beta Production

The ability of several estrogens and antiestrogens to activate PI-9 gene expression was assessed by transient transfection. Of interest, the mixed agonist/antagonist tamoxifen (OHT) was an agonist on the transiently transfected reporter gene but behaved as an antagonist on the cellular gene. To begin to assess how OHT-ER interacts with the native PI-9 promoter we carded out chromatin immunoprecipitation (ChIP) assays. The ChIP assays demonstrated that moxestrol-ER complex bound to the PI-9 promoter, but that OHT-ER (and unliganded ER) did not bind. These data provide the first evidence that the agonist activity of OHT-ER stems from the inability of OHT to elicit conformational changes in chromatin which allow ER to bind to ERE's.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Stobie-Krieg, Sacha Ann Skookum

Contributors dc:contributor

• Shapiro, David J.

• Biology, Molecular

Language dc:language

eng

Identifier

(MiAaPQ)AAI3023204

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/84779