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Showing 1 to 20 of 146 for “"Proteinase"”.
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Proteinase A und Schaumstabilität
Proteinasen verschiedener Proteinasefamilien zeigen eine negative Korrelation von Proteinaseaktivität und Abnahme der Schaumstabilität in Bier. Ein eindeutig negativer Einfluß auf die Schaumstabilität wurde sowohl für Proteinasen der Carboxylproteinasefamilie (Proteinase A und Pepsin), als auch der …
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A Ca²⁺-activated proteinase in chicken skeletal muscle
… rnM Ca²⁺), pH optimum (7.2) and sensitivity to proteinase inhibitors (inhibited by leupeptin and chymostatin). Both embryonic and adult enzymes were found to have molecular weights of 112000 daltons by gel filtration on Sephadex G-150. CAP activity was present in cultured skeletal muscle cells …
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Comparison of Murine Epididymal and Seminal Vesicle Proteinase Inhibitors.
Mammalian sperm must undergo a long and circuitous journey to reach their goal of fertilizing the ovum. This journey begins with sperm production in the seminiferous tubules of the testes.
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Effect of Temperature on Proteinase Production by Pseudomonas Species
Made available in DSpace on 2014-12-09T14:36:51Z (GMT). No. of bitstreams: 1 6507143.pdf: 2733727 bytes, checksum: d151dbf75cdbb25d6667801c2b52e056 (MD5) Previous issue date: 1965
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Proteinase 3 and Neutrophil Apoptosis in ANCA-Associated Systemic Vasculitis
ANCA-Associated Systemic Vasculitis (AASV) is characterized by leukocytoclasis- dying neutrophils surrounding small blood vessels. Previous studies have shown increased plasma PR3 and increased proportion of neutrophils expressing plasma membrane-associated PR3 (mPR3+). Neutrophils were isolated by …
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Estrogen Inducible Serpin, Proteinase Inhibitor 9, in Immunosurveillance and Apoptosis
The phytoestrogen genistein has attracted a great deal of recent interest. In MCF-7 cells, we find that 10 nM genistein induces PI-9 as effectively as saturating E2, and genistein treated MCF-7 cells are resistant to NK cell mediated cytotoxicity. In ERalphaHA cells, the dose response curves for …
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Isolation and characterization of cathepsin Z, a lysosomal cysteine proteinase
Cathepsin Z is a cysteine proteinase found in lysosomes of human cells. It was detected in human cultured cell lines using the peptidyl diazomethane inhibitor Fmoc-Leu-Leu- [¹²⁵]Tyr-CHN₂. The labeling of cathepsin Z by the inhibitor was both time- and concentration-dependent, and the proteinase was …
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The roles of proteinases and proteinase inhibitors in plant-nematode interactions
… this study was to investigate the roles of plant proteinase inhibitors in plant-nematode (Meloidogyne spp.) interactions. Transgenic tomato and tobacco plants were employed to examine the effects of proteinase inhibitor I or II transgene on nematode disease development. In the first part of this …
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Investigation of the Efficacy of Various Proteinase-Treated "Serpula (Treponema) Hyodysenteriae" Vaccines
… to enzymatic digestion of the following proteinases: Proteinase K, proteinase type X-15, proteinase type 2 fungal, papain proteinase, and pepsin proteinase. Sodium dodecyl sulphate polyacrylamide gel electrophoresis(SDS-PAGE) was performed on all bulk samples as well as control samples. …
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Estrogen Regulation of Proteinase Inhibitor 9 Gene Expression and Interleukin-1beta Production
The ability of several estrogens and antiestrogens to activate PI-9 gene expression was assessed by transient transfection. Of interest, the mixed agonist/antagonist tamoxifen (OHT) was an agonist on the transiently transfected reporter gene but behaved as an antagonist on the cellular gene. To …
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Modulators of Inflammation and Steroid Receptors Regulate Proteinase Inhibitor 9 Gene Expression
In addition to pro-inflammatory agents, the synthetic anti-inflammatory glucocorticoid, dexamethasone, induced PI-9 gene expression via an imperfect glucocorticoid response element (GRE) located at -208. Dexamethasone induced PI-9 mRNA and protein. Dexamethasone-glucocorticoid receptor (GR) complex …
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The immobilisation of Thermus strain Rt41A proteinase and its use in peptide synthesis
A proteinase isolated from Thermus strain Rt41A was purified to apparent homogeneity using ion exchange and FPLC chromatography and had a final specific activity of approximately 9000 azocasein units/mg. The substrate specificity of the proteinase against various peptide p-nitroanilide substrates …
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Estrogen Inducible Proteinase Inhibitor 9 Protects Target Cells From Immune Surveillance and Apoptosis
To test the effect of estrogen receptor (ER) ligands that induce PI-9 on TRAIL-mediated cytotoxicity, we used MCF-7, human breast cancer cells. In MCF-7 cells, induction of PI-9 by 17beta-estradiol (E2) inhibited TRAIL-mediated cytotoxicity. This is the first demonstration that regulation of PI-9 …
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Characterization of a potato Proteinase Inhibitor II gene whose expression in not wound-responsive
<p>A potato Proteinase Inhibitor II gene (pin2T) was isolated and characterized both at the molecular level and the functional level. The open reading frame as well as the 5[superscript]' and 3[superscript]' flanking regions were sequenced. The coding region and 3[superscript]' flanking region …
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The role of C-terminal cysteines in regulating human proteinase-activated receptor-1 function
Proteinase-activated receptors (PARs) are a novel group of G-protein coupled receptors (GPCRs). The most striking evidence to distinguish them from other GPCRs is that they carry their own tethered ligand within extracellular N-terminus. To activate the receptors, the tethered ligand is exposed by …
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Chemical synthesis, cloning and expression of a gene encoding systemin, a proteinase inhibitor-inducing factor
Wound-inducible proteinase inhibitors in plants elicit a defence mechanism by inactivating the proteinases of insects. This triggers a feedback mechanism causing overproduction of digestive enzymes together with a decrease in appetite, leading to starvation. System in, a polypeptide proteinase …
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The role of intracellular and extracellular cysteines in regulating human proteinase-activated receptor-2 (hPAR2) function
… G-protein coupled receptors termed Proteinase-Activated Receptors (PAR), has recently been implicated in cardiovascular disease. Previous pharmacological studies have found that activation of hPAR2 by mast cell tryptase, (the major PAR2 activator outside the gastrointestinal tract) …
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