{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/84779"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/84779","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Estrogen Regulation of Proteinase Inhibitor 9 Gene Expression and Interleukin-1beta Production","abstract":"The ability of several estrogens and antiestrogens to activate PI-9 gene expression was assessed by transient transfection. Of interest, the mixed agonist/antagonist tamoxifen (OHT) was an agonist on the transiently transfected reporter gene but behaved as an antagonist on the cellular gene. To begin to assess how OHT-ER interacts with the native PI-9 promoter we carded out chromatin immunoprecipitation (ChIP) assays. The ChIP assays demonstrated that moxestrol-ER complex bound to the PI-9 promoter, but that OHT-ER (and unliganded ER) did not bind. These data provide the first evidence that the agonist activity of OHT-ER stems from the inability of OHT to elicit conformational changes in chromatin which allow ER to bind to ERE's.","abstract_html":"The ability of several estrogens and antiestrogens to activate PI-9 gene expression was assessed by transient transfection. Of interest, the mixed agonist/antagonist tamoxifen (OHT) was an agonist on the transiently transfected reporter gene but behaved as an antagonist on the cellular gene. To begin to assess how OHT-ER interacts with the native PI-9 promoter we carded out chromatin immunoprecipitation (ChIP) assays. The ChIP assays demonstrated that moxestrol-ER complex bound to the PI-9 promoter, but that OHT-ER (and unliganded ER) did not bind. These data provide the first evidence that the agonist activity of OHT-ER stems from the inability of OHT to elicit conformational changes in chromatin which allow ER to bind to ERE's.","abstract_has_math":false,"creators":["Stobie-Krieg, Sacha Ann Skookum"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biochemistry","degree_department":null,"school":null,"contributors":["Shapiro, David J."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T22:27:51Z","date_published":"2015-09-25T22:27:51Z","updated_at":"2026-07-22T22:26:23Z","subjects":["Biology, Molecular"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3023204"],"render_values":[{"text":"(MiAaPQ)AAI3023204","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/84779","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Shapiro, David J."]},{"key":"dc:creator","label":"Author","values":["Stobie-Krieg, Sacha Ann Skookum"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T22:27:51Z","10000-01-01","2001"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Molecular"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/84779","(MiAaPQ)AAI3023204"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The ability of several estrogens and antiestrogens to activate PI-9 gene expression was assessed by transient transfection. Of interest, the mixed agonist/antagonist tamoxifen (OHT) was an agonist on the transiently transfected reporter gene but behaved as an antagonist on the cellular gene. To begin to assess how OHT-ER interacts with the native PI-9 promoter we carded out chromatin immunoprecipitation (ChIP) assays. The ChIP assays demonstrated that moxestrol-ER complex bound to the PI-9 promoter, but that OHT-ER (and unliganded ER) did not bind. These data provide the first evidence that the agonist activity of OHT-ER stems from the inability of OHT to elicit conformational changes in chromatin which allow ER to bind to ERE's.","Made available in DSpace on 2015-09-25T22:27:51Z (GMT). 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Of interest, the mixed agonist/antagonist tamoxifen (OHT) was an agonist on the transiently transfected reporter gene but behaved as an antagonist on the cellular gene. To begin to assess how OHT-ER interacts with the native PI-9 promoter we carded out chromatin immunoprecipitation (ChIP) assays. The ChIP assays demonstrated that moxestrol-ER complex bound to the PI-9 promoter, but that OHT-ER (and unliganded ER) did not bind. These data provide the first evidence that the agonist activity of OHT-ER stems from the inability of OHT to elicit conformational changes in chromatin which allow ER to bind to ERE's.","Made available in DSpace on 2015-09-25T22:27:51Z (GMT). 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