Characterization of Solution Species Relevant to the Peroxidase-Nadh Biochemical Oscillator

The discovery of significance of iron-NADH complexation for the kinetics of NADH decomposition and oxidation led to an investigation of the influence of solution oxygen and biologically prominent metal ions on NADH degradation. Almost all metal ions under investigation appeared to complex quickly with NADH. Studies of kinetics of simultaneous processes of NADH acid-catalyzed decomposition, complexation, and oxidation in different environments were undertaken. It was observed that Fe2+ and Fe3+ were the only solution ions which caused a decrease in NADH decomposition rates, opposite to all other metal ions under investigation. The presence of metal ions accelerated NADH acid-catalyzed decomposition to a greater extent than NADH oxidation. Contrary to most literature sources, it was determined that the second, and not the first step of decomposition is rate-limiting. (Abstract shortened by UMI.).

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Lvovich, Vadim Florentij

Contributors dc:contributor

• Dissertation Abstracts International, Volume

• Engineering, Chemical

Language dc:language

eng

Identifier

(MiAaPQ)AAI9904529

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/84413