Peptide Analysis Using Capillary Electrophoresis With Fluorescence Detection

Since the amino acids tryptophan, tyrosine and phenylalanine contain aromatic functionalities, they can be detected without derivatization when the proper ultraviolet excitation wavelengths are used. The application of wavelength-resolved CE-LIF for the native fluorescence detection of these amino acids and peptides that contain them is described for the classification of peptides based on their spectra. Preliminary results are presented for combining this method with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) for the identification of unknown peptides.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Oldenburg, Kurt Edward

Contributors dc:contributor

• Sweedler, Jonathan V.

• Chemistry, Biochemistry

Language dc:language

eng

Identifier

(MiAaPQ)AAI9834722

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/84401