{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/84401"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/84401","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Peptide Analysis Using Capillary Electrophoresis With Fluorescence Detection","abstract":"Since the amino acids tryptophan, tyrosine and phenylalanine contain aromatic functionalities, they can be detected without derivatization when the proper ultraviolet excitation wavelengths are used. The application of wavelength-resolved CE-LIF for the native fluorescence detection of these amino acids and peptides that contain them is described for the classification of peptides based on their spectra. Preliminary results are presented for combining this method with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) for the identification of unknown peptides.","abstract_html":"Since the amino acids tryptophan, tyrosine and phenylalanine contain aromatic functionalities, they can be detected without derivatization when the proper ultraviolet excitation wavelengths are used. The application of wavelength-resolved CE-LIF for the native fluorescence detection of these amino acids and peptides that contain them is described for the classification of peptides based on their spectra. Preliminary results are presented for combining this method with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) for the identification of unknown peptides.","abstract_has_math":false,"creators":["Oldenburg, Kurt Edward"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Sweedler, Jonathan V."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T22:14:18Z","date_published":"2015-09-25T22:14:18Z","updated_at":"2026-07-22T22:26:23Z","subjects":["Chemistry, Biochemistry"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI9834722"],"render_values":[{"text":"(MiAaPQ)AAI9834722","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/84401","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Sweedler, Jonathan V."]},{"key":"dc:creator","label":"Author","values":["Oldenburg, Kurt Edward"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T22:14:18Z","10000-01-01","1998"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry, Biochemistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI9834722","http://hdl.handle.net/2142/84401"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Since the amino acids tryptophan, tyrosine and phenylalanine contain aromatic functionalities, they can be detected without derivatization when the proper ultraviolet excitation wavelengths are used. The application of wavelength-resolved CE-LIF for the native fluorescence detection of these amino acids and peptides that contain them is described for the classification of peptides based on their spectra. Preliminary results are presented for combining this method with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) for the identification of unknown peptides.","Made available in DSpace on 2015-09-25T22:14:18Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9834722.pdf: 4834375 bytes, checksum: 3f5f519982473f11addb4af597370516 (MD5) Previous issue date: 1998","Embargo set by: Seth Robbins for item 85682 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","124 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1998."]},{"key":"dc:title","label":"Title","values":["Peptide Analysis Using Capillary Electrophoresis With Fluorescence Detection"]}]}],"canonical_facts":{"dc:contributor":["Sweedler, Jonathan V."],"dc:creator":["Oldenburg, Kurt Edward"],"dc:date":["2015-09-25T22:14:18Z","10000-01-01","1998"],"dc:description":["Since the amino acids tryptophan, tyrosine and phenylalanine contain aromatic functionalities, they can be detected without derivatization when the proper ultraviolet excitation wavelengths are used. The application of wavelength-resolved CE-LIF for the native fluorescence detection of these amino acids and peptides that contain them is described for the classification of peptides based on their spectra. Preliminary results are presented for combining this method with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) for the identification of unknown peptides.","Made available in DSpace on 2015-09-25T22:14:18Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9834722.pdf: 4834375 bytes, checksum: 3f5f519982473f11addb4af597370516 (MD5) Previous issue date: 1998","Embargo set by: Seth Robbins for item 85682 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","124 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1998."],"dc:identifier":["(MiAaPQ)AAI9834722","http://hdl.handle.net/2142/84401"],"dc:language":["eng"],"dc:subject":["Chemistry, Biochemistry"],"dc:title":["Peptide Analysis Using Capillary Electrophoresis With Fluorescence Detection"],"dc:type":["text"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:23Z"}