Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 90 for “"Fluorescence detection"”.
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Microfluidic-based Fluorescence Detection for Biomarker Analysis
… with the combination of microfluidics and fluorescence detection for biomarker analysis. The first platform described here is to isolate CTCs with high purity by using 2-stage sorting technique and herringbone features for aliquot stretching, including axial and lateral mixing to spread …
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Peptide Analysis Using Capillary Electrophoresis With Fluorescence Detection
… of wavelength-resolved CE-LIF for the native fluorescence detection of these amino acids and peptides that contain them is described for the classification of peptides based on their spectra. Preliminary results are presented for combining this method with matrix-assisted laser desorption …
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Large core polymer optical backplanes for fluorescence detection
Fluorescence based sensors are used for determining environmental parameters such as dissolved oxygen or pH in biological systems without disturbing a biological system's equilibrium. Recently, there has been a drive to provide biological analysis tools in a compact form, resulting in arrays of …
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Nanoscale protein analysis utilizing capillary electrophoresis and laser-induced fluorescence detection
… volume sampling and low protein concentration detection. Furthermore, one of the major trends in high throughput screening is the growing replacement of technologies that depend on radioactivity to generate a signal with those that rely on fluorescence. This trend towards non-radioactive …
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Single cell analysis by capillary electrophoresis with laser-induced native fluorescence detection
… by capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). This technique was chosen due to its high separation efficiencies, small sample volumes and sensitive detection. Native fluorescence was used, in which the analyte was not tagged with a fluorophore. 275-nm excitation …
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Neurotransmitter and Neuromodulator Analysis Using Capillary Electrophoresis With Laser -Induced Fluorescence Detection
A variety of results on these neurotransmitters/neuromodulators have been elucidated using CE-LIF methods. Experimental results of the D-Asp study are consistent with the hypothesis that D-Asp is involved in cell to cell communication in Aplysia californica. In addition, D-Glu is found in the …
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From practice to theory: computational studies on fluorescence detection and laser therapy in dermatology
… in the processes underlying laser treatments and fluorescence measurements. I apply this approach in the study of fluorescence detection and of laser therapy. First, I investigate three methods of fluorescence detection and the reported contrast between healthy skin and malignant tissue. I varied …
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ANALYSIS BY CAPILLARY ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE DETECTION: FROM SMALL MOLECULES TO WHOLE CELLS
Capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) is a versatile and sensitive analytical tool with many potential applications. Its advantages over other chromatographic methods include minimal solvent and sample requirements, low waste volumes, simple instrumentation, …
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Development of a Planar Capillary Electrophoresis device coupled with dual refractive index and fluorescence detection
… limitations remain for universal analyte detection and small footprint field deployable instruments. The objective of this thesis is to design a small-scale planar capillary electrophoresis (PCE) platform equipped with universal and fluorescence detection. This platform uses a short 10 cm …
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Wavelength-Resolved Fluorescence Detection in Capillary Electrophoresis: Application to Single -Cell Analysis of Monoamine Neurotransmitters
… to greatly expand the application of LINF detection in CE.
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Fluorescent Components in Human Urine. Characterization by High Performance Liquid Chromatography With Fluorescence Detection (Hplc)
… of the human urine matrix using a unique HPLC-fluorescence detection system. The detection system provided characterization information based on the results returned by a reverse file-search of trapped chromatographic peak excitation and emission spectra searched against a fluorescence library …
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Capillary and microchip gel electrophoresis using multiplexed fluorescence detection with both time-resolved and spectral-discrimination capabilities: applications in DNA sequencing using near-infrared fluorescence
… the development of a two-color, time-resolved fluorescence microscope for the acquisition of both steady-state and time-resolved data during capillary and microchip electrophoresis. The utility of this hybrid fluorescence detector has been demonstrated by applying it to DNA sequencing …
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Capillary electrophoresis with wavelength-resolved fluorescence for analysis of peptides
Wavelength-resolved fluorescence detection in capillary electrophoresis yields a powerful analytical tool for analysis of complex micro-environments. The design, development, and applications of wavelength-resolved fluorescence detection for capillary electrophoresis are reviewed.
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Polymer-Based Microfluidic Devices for High Throughput Single Molecule Detection: Applications in Biological and Drug Discovery
… suitable for optical imaging. Single-molecule detection (SMD) is another technology which has emerged to facilitate the realization of high throughput data processing afforded by its ability to eliminate sample processing steps and generate results with high statistical accuracy. A combination …
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Investigation of copper(II) complexes: I Copper(II) complexes of tryptophan and its analogues as postcolumn interaction components for indirect fluorescence detection of amino-containing analytes II High-performance liquid chromatographic separation of copper(II) azamacrocyclic complexes
… of tryptophan and its analogues for indirect fluorescence detection in high-performance liquid chromatography. First, indirect photometric and fluorometric detection in high performance liquid chromatography (HPLC) is reviewed, giving the functional definition of indirect detection employed …
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Evanescent field enhanced fluorescence on a photonic crystal surface
… of angles for more efficient collection by a fluorescence detection system. The multiplicative effects of enhanced excitation and enhanced photon extraction combine to provide improved signal-to-noise ratios for detection of fluorescent emitters, which in turn can be used to reduce the limits …
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Designing, construction and analysis of line-scanning detection instrument for photonic crystal enhanced fluorescence
… and analysis of a portable line-scanning detection instrument for photonic crystal enhanced fluorescence. In order to increase the fluorescence signal for low-concentration detection, we introduced the photonic crystal enhanced fluorescence detection modality and demonstrated that it is a …
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In Vitro Selection of Highly Efficient DNA Enzymes as RNA Nucleases and Metal Biosensors
… of 17E towards Pb2+ and the high sensitivity of fluorescence detection, 17E can measure the presence of Pb2+ ions over a concentration range of three orders of magnitude, with a detection limit as low as 10 nM. This system represents the first example of DNA-based biosensor for metal ions. …
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Structural Characterization of Liquid-Chromatographic Effluents via Reverse File-Searching of Fluorescence Spectra
… the technique of liquid chromatography, fluorescence detection and computerized file-searching to produce a new approach to mixture analysis. The technique operates by separating mixture components in a liquid-chromatograph and submitting them sequentially to a high-performance …
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Screening of algal cells using microdroplet technology
… cell suspension prior to the encapsulation. A fluorescence-activated droplet sorting platform was used to detect the fluorescence of encapsulated algal cells. Following modifications to the optics of a previously used platform, the fluorescence measurement uncertainty was reduced from 87% to …
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