Hybrid Gene Therapy Vectors Comprised of Synthetic Polymer and Noninfectious Virus -Like Particles

While the hybrid vectors have combined some of the beneficial traits of the polymer and viral components the efficiency of the vector is low, in comparison to conventional viral vectors. Based on the changes in gene expression over time and the effect of chloroquine, the limiting step of hybrid vector cell transduction appears to be endolysosomal escape of the complex. The polymer component fails to mediate escape as efficiently as the MLV envelope protein, likely resulting in the degradation of internalized hybrid vector complexes. Incorporation of polymers better suited for mediating escape from the endolysosomal network could likely improve the hybrid vector efficiency.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemical Engineering

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Ramsey, Joshua

Contributors dc:contributor

• Pack, Daniel W.

• Engineering, Chemical

Language dc:language

eng

Identifier

(MiAaPQ)AAI3250313

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/82392