Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 59 for “"envelope protein"”.
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Human coronavirus NL63 envelope protein-protein interactions: role in pathogenesis
… Of the four canonical coronavirus structural proteins, the envelope (E) protein remains the least well-characterised, despite its critical functions in viral assembly and release, as well as its role as a major contributor to viral pathogenesis. Its contribution to viral pathogenesis has been …
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Thioaptamers Targeting Dengue Virus Type-2 Envelope Protein Domain III
… fuses with the host cell membrane using surface envelope protein (E). The E protein ectodomain contains three domains, EDI, EDII and EDIII. The highly immunogenic EDIII is the putative receptor binding domain that also binds neutralizing antibodies. Thus, EDIII is an attractive target for …
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A Major Cell Envelope Protein Of Escherichia Coli K12: Purification And Properties
… isolation and characterization of a major cell envelope protein from E. coli K12, the so-called "succinate eluted protein" (SEP) is reported. SEP was released by EDTA-sucrose and osmotic shock treatments, or during the formation of spheroplasts. The protein was purified on columns of …
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Site-Specific Labelling of the Flavivirus Envelope Protein using Genetic Code Expansion
… a significant global public health burden. The envelope protein (E) orchestrates the processes of virus assembly, budding, and entry into cells and is the primary target of neutralising antibodies. The E proteins arranged on the surface of the virion are not static and pose a dynamic landscape …
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Phenotypic Characterization of Mutations Made to the Envelope Protein of West Nile Virus
… in death or long term neurological sequela. The envelope (E) protein of flaviviruses has three domains and is the major component of the virion surface, with the EIII being the putative receptor binding domain. Previous studies showed that Gly to Ala mutation of EIII residue 331 (G331A) yielded a …
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Structure-function of Dengue 2 virus envelope protein domain III with neutralizing antibodies
… from disease caused by the DENVs and the envelope (E) protein is the primary target. Potency of neutralization has been correlated with the particular domain on the E protein recognized by a neutralizing antibody, with domain III of the E protein (ED3) being the target of the most potent …
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Expression and Immunological Characterization of Herv-K Transmembrane Envelope Protein In Human Breast Cancer
… retrovirus K (HERV-K) <em>env</em> gene encodes envelope protein comprising surface (SU) and transmembrane (TM) domains. Having shown the exclusive expression of SU in human breast cancer and the stimulation of SU-specific immune responses in patients with breast cancer, our research here …
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Purification of the major envelope protein GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) from native virions
… heterogeneity. Evidence indicates that the major envelope protein, GP5, is the primary target for a subunit vaccine. In native virions GP5 primarily exists as a disulfide linked complex with the membrane protein, M, which also possesses immunogenic properties. Recent studies report that the GP5/M …
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Investigating the role of the West Nile virus and Koutango virus envelope protein in virulence phenotypes using structural protein chimeras
… able to study the contribution of an entire protein in the context of a replicative virus. The generation of chimeric flaviviruses bridges this gap by being able to study the contributions of a single protein or domain in the context of a replicative virus. Previous work by McAuley et al. …
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Evaluation of a Tetravalent DNA Vaccine against Dengue: Integrating Biochemical Studies on Dengue Virus Envelope Protein to a Domain-Based Antigen Design.
… using alternative approaches. Given that the envelope glycoprotein (E) is the main target of neutralizing antibodies, it has been used as the antigen of choice for vaccine development efforts. Here we present a detailed analysis of factors involved in the expression, secretion and folding of …
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GLYCOPEPTIDE ANALYSIS OF HIV-1 ENVELOPE PROTEIN USING HPLC/ESI-FTICR MS AND MALDI-TOF MS WITH ASIALOFETUIN ENRICHMENT AND SEPARATION TECHNIQUES
Glycosylation on proteins serve many biological roles and their heterogeneity can affect various biological functions. The site occupancy of the glycans as well as their various glycoforms makes glycans unique to analyze due to the fact that the variation in structure is not based on a genomic …
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Manipulation of the Moloney Murine Leukemia Virus Envelope Protein in an Effort to Develop Directly and Indirectly Targeted Retroviral Vectors for Use in Human Gene Therapy
… has been developed. The design of this chimeric envelope protein has the potential to make the insertion of varied receptor binding sites a simple and efficient process. The goal of my studies was to learn more about the potential of this design by taking steps towards challenging its ligand …
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Molecular characterization of the major envelope protein of porcine reproductive and respiratory syndrome virus (PRRSV) and evaluation of its use for a diagnostic assay, vaccine development, and the examination of quasispecies evolution
… reading frame 5 (ORF5) gene encoding the major envelope protein (GP5) of acute PRRSV isolates was characterized. Sequence and phylogenetic analyses revealed that seven of the acute PRRS virus (PRRSV) isolates were related to other N. American PRRSV isolates while one isolate, 98-37120-2, was …
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Hybrid Gene Therapy Vectors Comprised of Synthetic Polymer and Noninfectious Virus -Like Particles
… to mediate escape as efficiently as the MLV envelope protein, likely resulting in the degradation of internalized hybrid vector complexes. Incorporation of polymers better suited for mediating escape from the endolysosomal network could likely improve the hybrid vector efficiency.
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Impact of West Nile Virus Envelope Domain III Surface Loop Residues on Antigenicity and Virulence
… between birds and mosquitoes. Domain III of its envelope protein is the target of highly specific and potently neutralizing antibodies, and is thought to bind the host cell receptors. To determine the role of individual surface loop residues in the function and antigenicty of WNV EIII, a panel of …
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Impact of West Nile Virus Envelope Domain III Surface Loop Residues on Antigenicity and Virulence
… between birds and mosquitoes. Domain III of its envelope protein is the target of highly specific and potently neutralizing antibodies, and is thought to bind the host cell receptors. To determine the role of individual surface loop residues in the function and antigenicty of WNV EIII, a panel of …
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The study of highly pathogenic emerging zoonotic virus envelope proteins through pseudotyped virus generation
… viruses (PVs), incorporating the viral envelope protein of interest which constitutes the primary surface antigen. This permits the serological detection of neutralising antibodies without the need to handle live virus, as well as other viral entry studies. Hence, PVs are increasingly …
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Correlated long-distance dynamics modulate monoclonal antibody binding resistance in flaviviral envelope pretein domain-3: A molecular dynamics simulations study
… resistant mutations have been localized to the envelope protein domain-3 (ED3) of flaviviruses. Previously it was shown that regions constituting antibody binding sites of dengue-2 (DEN2) and West Nile virus (WNV) ED3 were energetically coupled with the interior of the protein. Protein-protein …
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Structure and function of glutamate transporters
… was computational modeling of two other proteins: the HIV gp120 envelope protein (gp120) and NAD(P)H:Quinone Oxidoreductase 1 (NQO1). The study of gp120 involved a statistical analysis of substitutions that occur during early infection of clade A HIV, showing that during early infection …
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