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University of Illinois at Urbana-Champaign

Lactose Operon Expression and Plasmid Rearrangement in Rhodopseudomonas Sphaeroides (Gram Negative Bacteria)

Abstract

dc:description

Genetic tools were developed to study gene regulation in Rhodopseudomonas sphaeroides. In initial studies expression of the transposable lac operon, Tn951, was examined. Very low levels of (beta)-galactosidase were observed in R. sphaeroides but mutants of this bacterium were recovered that were able to use lactose as the sole carbon source and which had elevated (beta)-galactosidase expression. The mutation allowing growth on lactose was determined to be on the R. sphaeroides endogenote and not on Tn951 DNA.

Degree

thesis:*
Name thesis:degree_name
Ph.D.
Level thesis:degree_level
Dissertation
Discipline thesis:degree_discipline
Microbiology
Grantor
University of Illinois at Urbana-Champaign
Year dc:date
2014

Author and committee

dc:creator, dc:contributor.*
Author dc:creator
  • Nano, Francis Edward

Subjects

dc:subject × 1

Identifiers

dc:identifier.*
Identifier
(UMI)AAI8409815
OAI identifier oai:identifier
oai:www.ideals.illinois.edu:2142/71160

Chain of custody

source
Harvested from
University of Illinois - Urbana-Champaign
Base URL
www.ideals.illinois.edu/oai-pmh
Last updated
2026-07-22
Source record
OAI-PMH GetRecord
citation

Nano, Francis Edward. Lactose Operon Expression and Plasmid Rearrangement in Rhodopseudomonas Sphaeroides (Gram Negative Bacteria). Dissertation thesis, University of Illinois at Urbana-Champaign, 2014. http://hdl.handle.net/2142/71160