University of Illinois at Urbana-Champaign
Lactose Operon Expression and Plasmid Rearrangement in Rhodopseudomonas Sphaeroides (Gram Negative Bacteria)
Abstract
dc:descriptionGenetic tools were developed to study gene regulation in Rhodopseudomonas sphaeroides. In initial studies expression of the transposable lac operon, Tn951, was examined. Very low levels of (beta)-galactosidase were observed in R. sphaeroides but mutants of this bacterium were recovered that were able to use lactose as the sole carbon source and which had elevated (beta)-galactosidase expression. The mutation allowing growth on lactose was determined to be on the R. sphaeroides endogenote and not on Tn951 DNA.
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Microbiology
- Grantor
- University of Illinois at Urbana-Champaign
- Year dc:date
- 2014
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Nano, Francis Edward
Subjects
dc:subject × 1Identifiers
dc:identifier.*- Identifier
- (UMI)AAI8409815
- OAI identifier oai:identifier
- oai:www.ideals.illinois.edu:2142/71160