{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/71160"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/71160","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Lactose Operon Expression and Plasmid Rearrangement in Rhodopseudomonas Sphaeroides (Gram Negative Bacteria)","abstract":"Genetic tools were developed to study gene regulation in Rhodopseudomonas sphaeroides. In initial studies expression of the transposable lac operon, Tn951, was examined. Very low levels of (beta)-galactosidase were observed in R. sphaeroides but mutants of this bacterium were recovered that were able to use lactose as the sole carbon source and which had elevated (beta)-galactosidase expression. The mutation allowing growth on lactose was determined to be on the R. sphaeroides endogenote and not on Tn951 DNA.","abstract_html":"Genetic tools were developed to study gene regulation in Rhodopseudomonas sphaeroides. In initial studies expression of the transposable lac operon, Tn951, was examined. Very low levels of (beta)-galactosidase were observed in R. sphaeroides but mutants of this bacterium were recovered that were able to use lactose as the sole carbon source and which had elevated (beta)-galactosidase expression. The mutation allowing growth on lactose was determined to be on the R. sphaeroides endogenote and not on Tn951 DNA.","abstract_has_math":false,"creators":["Nano, Francis Edward"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-16T06:12:50Z","date_published":"2014-12-16T06:12:50Z","updated_at":"2026-07-22T22:26:04Z","subjects":["Biology, Microbiology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8409815"],"render_values":[{"text":"(UMI)AAI8409815","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/71160","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Nano, Francis Edward"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-16T06:12:50Z","10000-01-01","1984"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Microbiology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/71160","(UMI)AAI8409815"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Genetic tools were developed to study gene regulation in Rhodopseudomonas sphaeroides. In initial studies expression of the transposable lac operon, Tn951, was examined. Very low levels of (beta)-galactosidase were observed in R. sphaeroides but mutants of this bacterium were recovered that were able to use lactose as the sole carbon source and which had elevated (beta)-galactosidase expression. The mutation allowing growth on lactose was determined to be on the R. sphaeroides endogenote and not on Tn951 DNA.","In an effort to experimentally approach an understanding of transcriptional regulation in R. sphaeroides Mu dl(Ap('R), lac) was introduced via the R-plasmid, R751. The introduction of Mu dl(Ap('R), lac) was found to enhance the appearance of photosynthetic deficient mutants of R. sphaeroides; Mu dl(Ap('R), lac) DNA was found not to be physically associated with endogenote DNA. In many of the photosynthetic mutants the indigenous R. sphaeroides plasmid were found to be rearranged. It was further discovered that the plasmids involved in the rearrangements had extensive DNA homology.","In another approach to understanding gene regulation in R. sphaeroides a broad host range plasmid was constructed that permitted the translational fusion of target DNA to the lac operon. R. sphaeroides DNA was cloned into this plasmid and recombinant plasmids were introduced into R. sphaeroides. Several isolates were recovered that produced various levels of (beta)-galactosidase, presumably representing the cloning of various R. sphaeroides promoters.","Made available in DSpace on 2014-12-16T06:12:50Z (GMT). No. of bitstreams: 1 8409815.pdf: 4282947 bytes, checksum: dbcebab84096308f7bf587c49cf6251d (MD5) Previous issue date: 1984","Embargo set by: Seth Robbins for item 71326 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","178 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1984."]},{"key":"dc:title","label":"Title","values":["Lactose Operon Expression and Plasmid Rearrangement in Rhodopseudomonas Sphaeroides (Gram Negative Bacteria)"]}]}],"canonical_facts":{"dc:creator":["Nano, Francis Edward"],"dc:date":["2014-12-16T06:12:50Z","10000-01-01","1984"],"dc:description":["Genetic tools were developed to study gene regulation in Rhodopseudomonas sphaeroides. In initial studies expression of the transposable lac operon, Tn951, was examined. Very low levels of (beta)-galactosidase were observed in R. sphaeroides but mutants of this bacterium were recovered that were able to use lactose as the sole carbon source and which had elevated (beta)-galactosidase expression. The mutation allowing growth on lactose was determined to be on the R. sphaeroides endogenote and not on Tn951 DNA.","In an effort to experimentally approach an understanding of transcriptional regulation in R. sphaeroides Mu dl(Ap('R), lac) was introduced via the R-plasmid, R751. The introduction of Mu dl(Ap('R), lac) was found to enhance the appearance of photosynthetic deficient mutants of R. sphaeroides; Mu dl(Ap('R), lac) DNA was found not to be physically associated with endogenote DNA. In many of the photosynthetic mutants the indigenous R. sphaeroides plasmid were found to be rearranged. It was further discovered that the plasmids involved in the rearrangements had extensive DNA homology.","In another approach to understanding gene regulation in R. sphaeroides a broad host range plasmid was constructed that permitted the translational fusion of target DNA to the lac operon. R. sphaeroides DNA was cloned into this plasmid and recombinant plasmids were introduced into R. sphaeroides. Several isolates were recovered that produced various levels of (beta)-galactosidase, presumably representing the cloning of various R. sphaeroides promoters.","Made available in DSpace on 2014-12-16T06:12:50Z (GMT). No. of bitstreams: 1 8409815.pdf: 4282947 bytes, checksum: dbcebab84096308f7bf587c49cf6251d (MD5) Previous issue date: 1984","Embargo set by: Seth Robbins for item 71326 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","178 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1984."],"dc:identifier":["http://hdl.handle.net/2142/71160","(UMI)AAI8409815"],"dc:subject":["Biology, Microbiology"],"dc:title":["Lactose Operon Expression and Plasmid Rearrangement in Rhodopseudomonas Sphaeroides (Gram Negative Bacteria)"],"dc:type":["text"],"thesis:degree_discipline":["Microbiology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:04Z"}