Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 347 for “"operon"”.
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Nitrogen regulation of theput operon in enteric bacteria
… Expression of the proline utilization (put) operon in Klebsiella aerogenes and Escherichia coli is responsive to nitrogen regulation, but Salmonella typhimurium can not activate put operon expression when growing in medium with glucose as a carbon source and proline as the sole nitrogen …
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Negative Regulation of the accBC Operon in Escherichia Coli
… and caused negative regulation of the accBC operon. Complementation of ACC activity using the malonyl-CoA synthase gene (matB) from Rhizobium trifolii allowed the deletion of the accA and accB genes. Transcriptional activity of the accBC operon in this strain shows an absence of growth-rate …
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Transcriptional regulation of the Bacillus subtilis pyrimidine biosynthetic operon
… complete transcript of the Bacillus subtilis pyr operon contains the following elements in 5$\sp\prime$ to 3$\sp\prime$ order: a 150 nt untranslated leader; pyrR, which encodes a 21 kDa protein; a 173 nt intercistronic region; pyrP, which encodes a 46 kDa protein; a 145 nt intercistronic region; …
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Molecular biology studies on thecyd operon of Escherichia coli
… studies indicate that cyd is a two-cistron operon with the subunit I gene upstream of the subunit II gene. S1 nuclease mapping experiments were used to show that the initiation of transcription occurs at $-$287 bases upstream of the start codon of the subunit I gene. In order to define the …
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Análisis genético y molecular del operon std de salmonella enterica
Salmonella enterica serovar Typhimurium es una bacteria gram-negativa que causa enfermedades gástricas y sistémicas en una gran variedad de organismos, incluyendo la especie humana. La invasión del epitelio intestinal es un proceso crítico en la infección por Salmonella y requiere funciones …
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Characterization of the glpEGR operon of Escherichia coli K-12
… reading frames (ORF) are present in the glpEGR operon. The glpR-encoded repressor governs the universal negative regulation of the glp regulon. The glpE and glpG genes are located upstream of glpR. The initiation codons as well as the translational reading frames used by these two genes were …
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Investigation of a ribosomal RNA operon of Frankia sp. strain AcN14a.
With the aim of increasing our plant knowledge of Frankia, we have investigated the structure of the ribosomal RNA genes from Frankia sp. strain AcN14a. A genomic library was constructed in EMBL3 and two types of clones, each containing a different set of rRNA genes, were identified by restriction …
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Regulation of the Mannose PTS Operon by the small RNA SgrS
A number of bacterial small RNAs (sRNAs) act as global regulators of stress responses by controlling expression of multiple genes. The sRNA SgrS is expressed in response to glucose-phosphate stress, a condition associated with disruption of glycolytic flux and accumulation of sugar-phosphates. SgrS …
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Lactose Operon Expression and Plasmid Rearrangement in Rhodopseudomonas Sphaeroides (Gram Negative Bacteria)
… studies expression of the transposable lac operon, Tn951, was examined. Very low levels of (beta)-galactosidase were observed in R. sphaeroides but mutants of this bacterium were recovered that were able to use lactose as the sole carbon source and which had elevated (beta)-galactosidase …
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Molecular genetic analysis of puf andpuc operon expression in Rhodobacter sphaeroides 2.4.1
The deletion of the puf intercistronic terminator resulted in both the loss of the smallest 0.5-kilobase (kb) puf transcript in addition to an approximate 10-fold increase in transcriptional read-through of the mutated region to more distal pufL gene, suggesting that the proximal intercistronic …
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THE SUBTLE BIOFILM REGULATION IN ESCHERICHIA COLI: CSGD AND THE YDDV-DOS OPERON
… et al., 2006; Weber et al., 2002). The yddV-dos operon is the most expressed among c-di-GMP-related genes showing dependence on σs (Weber et al., 2006; Sommerfeldt et al., 2009). It encodes, respectively, a protein with DGC activity and a PDE that can degrade c-di- GMP to pGpG. Both Dos and YddV …
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Protein -Dna Interactions Required for Regulation of the Proline Utilization Operon in Salmonella
A modification of the S&barbelow;ystematic E&barbelow;volution of L&barbelow;igands by Exponential Enrichment (SELEX) approach was used to enrich for put control DNA molecules defective in PutA binding in vitro. These experiments identified nucleotide residues that may be important for the …
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Molecular Characterization of the Heat Shock-Regulatedgroesl Operon in Lactobacillus Zeae Strain 102S
… heat induction conditions for the groESL operon were determined by Northern and slot blot analyses. Changes in the level of the molecular chaperone GroEL (HSP 60) were analyzed by Western blotting and found to correlate well with the changes in RNA expression levels. The specific promoter …
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Genetic studies on the region downstream of the unc operon of Thiobacillus ferroxidans
… from p818.1 downstream the T ferrooxidans unc operon and sequenced in both directions. One partial open reading frame (ORFl) and two complete open reading frames (ORF2 and ORF3) were found. On the basis of high homology to previously published sequences, ORFI was found to be the C-terminus of …
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An Investigation of Acetobacter aceti N5-Carboxyaminoimidazole Ribonucleotide Mutase and Its purE-purK Operon
<italic>Acetobacter aceti</italic> oxidizes ethanol to acetic acid. While the membrane-permeable acetic acid is toxic to many bacteria, A. aceti survives exposure to acetic acid by tolerating cytoplasmic acidification. The ability to tolerate an acidic cytoplasm suggests that proteins from A. aceti …
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