Effects of stiffness gradient and hydrostatic pressure on cell migratory behaviors

This thesis explores the effects of mechanical stimuli on cell patterning and behavior, which may influence our understanding of in vivo processes such as morphogenesis, wound healing, disease progression, tumor formation, and metastatic events. We begin by looking at the effects of a stiffness gradient in three-dimensional culture environment, which builds upon prior research on the effects of stiffness in a two-dimensional culture, but provides a platform for studying these outcomes that is more akin to the in vivo environment. Then we look at the effect of hydrostatic pressure using a non-contact pressure chamber that provides a uniform pressure environment while maintaining physiological conditions needed for cell growth. By seeking to better understand the migratory responses of cells to various mechanical stimuli, we gain tools and knowledge that can be used to aid in diagnoses and treatment of a variety of diseases.

Name thesis:degree_name

M.S.

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Mechanical Engineering

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Joaquin, Danielle

Contributors dc:contributor

• Hsia, K. Jimmy

• Mechanobiology
• Tissue Engineering
• Biological stiffness gradient
• Cell migration
• Cell motility
• Cellular organization
• Pressurized incubation chamber
• Scratch Assay

Statement dc:rights

• Copyright 2014 Danielle Joaquin

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/49651

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/49651