Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"Scratch Assay"”.
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Establishing a structure-activity-relationship for small molecule non-sugar glycomimetics
… potency against wound healing in DAOY cells in a scratch assay against human growth factor/scatter factor (HGF/SF) as a control. Compounds trialled in scratch assays did not show any reasonable biological activity. HGF/SF has been shown to activate downstream signalling of the oncogene product …
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An immunohistopathological and functional investigation of β3 integrin antagonism as a therapeutic strategy in cancer. Characterisation, development, and utilisation of preclinical cancer models to investigate novel ¿3 integrin anatgonists.
… a functional test for cell migration, the scratch wound healing assay. Migration of tumour cells that expressed αVβ3 integrin was inhibited by the known β3 antagonists, cRGDfV peptide and LM609 antibody. A panel of 12 potential novel β3 integrin antagonists was screened for cytotoxicity and …
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PTEN loss induces epithelial-mesenchymal transition in human colon cancer cells
… was measured using a modified Boyden chamber assay and migration was assessed using a scratch assay. Results. PTEN knockdown increases the invasion and migration of CRC cells and the addition of medium containing TNF-á further enhanced the migration and invasion. PTEN knockdown resulted in …
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Evaluation of new succinimide-based small molecules as alphavbeta3 integrin antagonists: from design to preliminary biological testing
… molecules have been tested in ex-vivo metabolism assay in liver, kidney and tumour tissues. Cytotoxicity has been tested for all compounds in M14 and DLD1 cell lines: no effect was observed in MTT assay. Migration inhibition has been evaluated by performing scratch assay on M14 and DLD1 cell …
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Design, Synthesis and Biological Evaluation of Inhibitors of Polysialyltransferases PST and STX. Design, synthesis and biological evaluation of a range of N-modified mannosamines, sialic acids and analogues from in silico screening as inhibitors of PolySia-NCAM biosynthesis with anti-migration activity.
… active compounds are described. Using an enzyme assay, compounds were evaluated for their ability to reduce polySia synthesis through polyST inhibition. Effects of agents on polySia expression in cells, and the ability of compounds to reduce cell migration in vitro was studied using a wound …
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Effect of Anti-fibrotic Drug (Pirfenidone) on TGF-β Signaling Pathway in Ocular Fibrotic Disorders
… was measured with enzyme-linked immunosorbent assay, and cell migration activity was investigated using a scratch assay. Immunoblot analyses of cofilin, Smad protein (Smad) 2/3, p38 mitogen-activated protein kinase, c-Jun N-terminal kinase, and extracellular signal related kinase expression …
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Yap and Taz Are Required For Neural Crest-Derived Cardiovascular Development
… data together with transwell migration assay, scratch assay, and <em>ex vivo </em>culture, indicate a potential role for <em>Yap/Taz</em> in regulating NC migration. Together, our data indicate that <em>Yap/Taz</em> play a critical role in proper cardiac NC-derived cardiac formation.</p>
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The Role of Interleukin-10 Family Members in Inflammatory Skin Diseases. Understanding the mechanism of action of interferon lambda and interleukin-22 on human primary keratinocytes and dermal fibroblasts with a focus on healing responses in inflammatory skin diseases
… in skin resident cells using cell trace dye and scratch assay. Dermal fibroblasts were assessed for the presence of IFNλR1 and IL-22R1, downstream activities of the receptors. Results showed that IL-22 accelerated “scratch” closure in keratinocytes while IFNλ caused a delay in closure. IL-22 …
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The effect of synthetic cannabinoids on wound healing of chondrocytes monolayers and pseudo 3D cartilage tissue. Effect of different concentrations of synthetic cannabinoids WIN55, 212-2, URB602 and HU-308 with and without their antagonists on wound healing of chondrocyte monolayers and pseudo 3D cartilage tissue.
… monolayers was investigated using a simple scratch assay model. The three cannabinoids were found to increase wound healing of chondrocyte monolayers, but at different rates. WIN55, 212-2 at a concentration of 1μM had the highest effect of increasing both migration and proliferation of …
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Epidermal growth factor and Transforming growth factor α motogenic activities are mediated by Epidermal growth factor receptor : Identification of signalling pathways involved in head and neck cancer
… the stimulatory effect of EGF and TGFα in these assays.<b><br/></b><b><br/></b><b>Methods</b>: Three tumour cell lines of Asian origin were used in the study and a normal skin keratinocyte as a control. Cell proliferation was measured using an automated cell counting system. Migration was studied …
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Effect of Diabetes Mellitus on Ocular Surface Tight Junctions and Glycocalyx
… Trans-epithelial electrical resistance and scratch assay were performed to quantify barrier functions and cell migration. Gene and protein expression of tight junction proteins: claudin-1, claudin-2, claudin-3, ZO-1, ZO-2, ZO-3, and occludin was quantified using real time PCR and western …
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The Physiological and Genetic Factors Underpinning the Response to Muscle Damaging Exercise
… repair/recovery after an artificial wounding (scratch) assay using of primary human skeletal muscle cells in vitro; (iii) to ascertain whether multiple genetic variations, which are linked to varying tissues, forming a polygenic profile could distinguish between high and low responders …
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Developing a single dressing containing both antifungal and antibacterial drugs for treating mixed infections in diabetic foot ulcers
… and showed maximum wound closure (in vitro scratch assay) within 7 days. CA wafers and films appeared to be potential delivery systems for delivering antimicrobial agents to mixed infected DFUs.
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The role of the kisspeptin receptor, KISS1R, in breast cancer cell proliferation, invasion and migration.
… were assessed using crystal violet staining and scratch assay, respectively. Lastly, the last exon, exon 5 of the KISS1R gene in the BT-20 and MDA-MB-231 cell lines was assessed by Sanger sequencing. The results show that KISS1R protein is expressed in all the breast cancer cell lines tested …
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Investigation of the beta 2 adrenergic receptor (B2-AR) pathway in canine hemangiosarcoma
… Cell proliferation was measured through MTS assay, which revealed an increase in cell proliferation only in Fitz cells treated with 0.1 μM of NE (25% increase) and cells treated with 0.1 μM of propranolol (30% increase). Cell migration was evaluated with a scratch assay and was decreased only …
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An investigation into the cellular mechanisms underlying photodynamic rejuvenation in human skin
… was indeed occurring after the therapy, a ROS assay was performed. The yellow laser and UVA transilluminator, which emit light maximally absorbed by hypericin, were used. UVA served as the upper limit for ROS generation as this range of wavelengths is known to cause intracellular ROS. Yellow …
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Development and Characterization of Selective CB2R Inverse Agonists as a Novel Anti-Inflammatory Therapy for Neuroinflammation – Mechanisms of Action in Murine and Human Microglia Models Provide Valuable Insights into Their Therapeutic Potential
… and evaluated in ACTOne and TANGO-bla functional assays for CB1 and CB2 activity in combination with affinity measurements for the CB1/2 receptors. Moreover, the compounds that demonstrated no activity at functional assays with high binding affinities to CB2R were further assessed in antagonist …
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Studio degli effetti benefici dei functional foods Lycium barbarum e Brassica oleracea su modelli cellulari ed approcci in vivo
La conoscenza delle basi biochimiche delle malattie ha indotto a verificare l ipotesi di poter modulare le funzioni cellulari e potenziare i sistemi di difesa dell’organismo attraverso composti bioattivi presenti negli alimenti naturali, scoprendo e valorizzando la componente funzionale di alimenti …
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Effects of stiffness gradient and hydrostatic pressure on cell migratory behaviors
This thesis explores the effects of mechanical stimuli on cell patterning and behavior, which may influence our understanding of in vivo processes such as morphogenesis, wound healing, disease progression, tumor formation, and metastatic events. We begin by looking at the effects of a stiffness …