Hepatic gene networks due to the trans-10, cis-12-conjugated linoleic acid supplementation in growing male mice

Trans-10, cis-12-conjugated linoleic acid (trans-10, cis-12-CLA) has been reported to reduce body fat mass but accompanied by increased liver mass. However, the cellular and molecular mechanisms have been unknown. Our study investigated the effects of trans-10, cis-12-CLA supplementation on hepatic gene expression in growing male mice by measuring mRNA abundance of thirty-eight genes encoding important enzymes, ligand-dependent nuclear receptors, transcription regulators and transporters in lipid metabolism. Feeding growing male mice diets supplemented with 0.3% trans-10, cis-12-CLA for 6 weeks significantly increased the expression of hepatic genes involved in fatty acid uptake (Cd36), TG synthesis (Acaca, Gpam and Scd), lipid droplet formation (Plin2), VLDL assembling/secretion (Mttp and Cideb), ketogenesis and utilization (Hmgcs2 and Bdh1), fatty acid oxidation (Acox1), glyceroneogenesis (Pck1 and Pdk4). The results suggest that the increased liver weight in response to trans-10, cis-12-CLA may be due to increased fatty acid uptake, stimulated TG synthesis, enhanced formation of lipid droplets as well as insufficiently induced VLDL assembly/secretion mechanism in liver. We provided new evidences to advance in the knowledge of the effect of this supplement in a relatively low amount on hepatic gene expression in growing male mice.

Name thesis:degree_name

M.S.

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Nutritional Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Li, Jing

Contributors dc:contributor

• Loor, Juan J.

• Conjugated linoleic acid
• hepatic steatosis

Statement dc:rights

• Copyright 2011 Jing Li

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/24310

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/24310