{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/24310"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/24310","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Hepatic gene networks due to the trans-10, cis-12-conjugated linoleic acid supplementation in growing male mice","abstract":"Trans-10, cis-12-conjugated linoleic acid (trans-10, cis-12-CLA) has been reported to reduce body fat mass but accompanied by increased liver mass. However, the cellular and molecular mechanisms have been unknown. Our study investigated the effects of trans-10, cis-12-CLA supplementation on hepatic gene expression in growing male mice by measuring mRNA abundance of thirty-eight genes encoding important enzymes, ligand-dependent nuclear receptors, transcription regulators and transporters in lipid metabolism. Feeding growing male mice diets supplemented with 0.3% trans-10, cis-12-CLA for 6 weeks significantly increased the expression of hepatic genes involved in fatty acid uptake (Cd36), TG synthesis (Acaca, Gpam and Scd), lipid droplet formation (Plin2), VLDL assembling/secretion (Mttp and Cideb), ketogenesis and utilization (Hmgcs2 and Bdh1), fatty acid oxidation (Acox1), glyceroneogenesis (Pck1 and Pdk4). The results suggest that the increased liver weight in response to trans-10, cis-12-CLA may be due to increased fatty acid uptake, stimulated TG synthesis, enhanced formation of lipid droplets as well as insufficiently induced VLDL assembly/secretion mechanism in liver. We provided new evidences to advance in the knowledge of the effect of this supplement in a relatively low amount on hepatic gene expression in growing male mice.","abstract_html":"Trans-10, cis-12-conjugated linoleic acid (trans-10, cis-12-CLA) has been reported to reduce body fat mass but accompanied by increased liver mass. However, the cellular and molecular mechanisms have been unknown. Our study investigated the effects of trans-10, cis-12-CLA supplementation on hepatic gene expression in growing male mice by measuring mRNA abundance of thirty-eight genes encoding important enzymes, ligand-dependent nuclear receptors, transcription regulators and transporters in lipid metabolism. Feeding growing male mice diets supplemented with 0.3% trans-10, cis-12-CLA for 6 weeks significantly increased the expression of hepatic genes involved in fatty acid uptake (Cd36), TG synthesis (Acaca, Gpam and Scd), lipid droplet formation (Plin2), VLDL assembling/secretion (Mttp and Cideb), ketogenesis and utilization (Hmgcs2 and Bdh1), fatty acid oxidation (Acox1), glyceroneogenesis (Pck1 and Pdk4). The results suggest that the increased liver weight in response to trans-10, cis-12-CLA may be due to increased fatty acid uptake, stimulated TG synthesis, enhanced formation of lipid droplets as well as insufficiently induced VLDL assembly/secretion mechanism in liver. We provided new evidences to advance in the knowledge of the effect of this supplement in a relatively low amount on hepatic gene expression in growing male mice.","abstract_has_math":false,"creators":["Li, Jing"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Nutritional Sciences","degree_department":null,"school":null,"contributors":["Loor, Juan J."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-25T14:50:57Z","date_published":"2011-05-25T14:50:57Z","updated_at":"2026-07-22T22:25:23Z","subjects":["Conjugated linoleic acid","hepatic steatosis"],"languages":["en"],"rights":["Copyright 2011 Jing Li"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/24310","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Loor, Juan J."]},{"key":"dc:creator","label":"Author","values":["Li, Jing"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-25T14:50:57Z","2011-05"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Nutritional Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Conjugated linoleic acid","hepatic steatosis"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2011 Jing Li"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/24310"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Trans-10, cis-12-conjugated linoleic acid (trans-10, cis-12-CLA) has been reported to reduce body fat mass but accompanied by increased liver mass. However, the cellular and molecular mechanisms have been unknown. Our study investigated the effects of trans-10, cis-12-CLA supplementation on hepatic gene expression in growing male mice by measuring mRNA abundance of thirty-eight genes encoding important enzymes, ligand-dependent nuclear receptors, transcription regulators and transporters in lipid metabolism. Feeding growing male mice diets supplemented with 0.3% trans-10, cis-12-CLA for 6 weeks significantly increased the expression of hepatic genes involved in fatty acid uptake (Cd36), TG synthesis (Acaca, Gpam and Scd), lipid droplet formation (Plin2), VLDL assembling/secretion (Mttp and Cideb), ketogenesis and utilization (Hmgcs2 and Bdh1), fatty acid oxidation (Acox1), glyceroneogenesis (Pck1 and Pdk4). The results suggest that the increased liver weight in response to trans-10, cis-12-CLA may be due to increased fatty acid uptake, stimulated TG synthesis, enhanced formation of lipid droplets as well as insufficiently induced VLDL assembly/secretion mechanism in liver. We provided new evidences to advance in the knowledge of the effect of this supplement in a relatively low amount on hepatic gene expression in growing male mice.","Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2011-04-29T17:04:18Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Li_Jing.docx: 2056751 bytes, checksum: 4a050dd9069c2cc3bca64c68fab614d9 (MD5) Li_Jing.pdf: 1805956 bytes, checksum: 3c8bceedb69bb3c622f72010ce0be556 (MD5)","Made available in DSpace on 2011-05-25T14:50:57Z (GMT). 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Our study investigated the effects of trans-10, cis-12-CLA supplementation on hepatic gene expression in growing male mice by measuring mRNA abundance of thirty-eight genes encoding important enzymes, ligand-dependent nuclear receptors, transcription regulators and transporters in lipid metabolism. Feeding growing male mice diets supplemented with 0.3% trans-10, cis-12-CLA for 6 weeks significantly increased the expression of hepatic genes involved in fatty acid uptake (Cd36), TG synthesis (Acaca, Gpam and Scd), lipid droplet formation (Plin2), VLDL assembling/secretion (Mttp and Cideb), ketogenesis and utilization (Hmgcs2 and Bdh1), fatty acid oxidation (Acox1), glyceroneogenesis (Pck1 and Pdk4). The results suggest that the increased liver weight in response to trans-10, cis-12-CLA may be due to increased fatty acid uptake, stimulated TG synthesis, enhanced formation of lipid droplets as well as insufficiently induced VLDL assembly/secretion mechanism in liver. We provided new evidences to advance in the knowledge of the effect of this supplement in a relatively low amount on hepatic gene expression in growing male mice.","Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2011-04-29T17:04:18Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Li_Jing.docx: 2056751 bytes, checksum: 4a050dd9069c2cc3bca64c68fab614d9 (MD5) Li_Jing.pdf: 1805956 bytes, checksum: 3c8bceedb69bb3c622f72010ce0be556 (MD5)","Made available in DSpace on 2011-05-25T14:50:57Z (GMT). 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