Isolation, characterization and expression of a peach nuclear gene encoding a light-harvesting chlorophyll a/b-binding polypeptide of photosystem II

A nuclear gene encoding a light-harvesting chlorophyll a/b-binding polypeptide of photosystem II has been isolated from a peach genomic library (Prunus persica L. cv. Stark Earliglo) and characterized. The genomic library was constructed and screened with a (α-$\sp{32}$P) -labeled pea Lhcb1*1 cDNA probe for positive phage containing Lhcb gene sequences. A phage with an Sstl-restricted peach DNA fragment containing an Lhcb gene sequence was identified. This DNA fragment was subcloned (pAB19) and sequenced completely in both directions. pAB19 contains a complete Lhcb gene coding sequence, designated Lhcb1*Pp1, as well as 5$\sp\prime$ and 3$\sp\prime$ flanking regions. Lhcb1*Pp1 encodes a precursor polypeptide of 267 amino acids composed of a transit peptide of 34 amino acids and a mature polypeptide of 233 amino acids. Transcription initiation and termination sites of Lhcb1*Pp1 mRNA were determined by S1 nuclease mapping. The cap site is located 70 bp upstream from the translation start site and 44 bp downstream from a putative TATA box. Multiple polyadenylation sites are present in the 3$\sp\prime$ flanking region. The seasonal expression of Lhcb1*Pp1 was determined by Northern blot and S1 nuclease protection analyses. Both analyses indicate that the relative level of Lhcb1*Pp1 mRNA per unit total leaf RNA declined to a small extent (27%) from the beginning (June) to the end (September) of the growing season, but decreased by 72% with senescence in October. Also, S1 nuclease protection analyses with 5$\sp\prime$ and 3$\sp\prime$ gene probes showed that Lhcb1*Pp1 is expressed in three varieties of P. persica and in light-grown but its expression is undetectable in dark-grown leaves and stems of the Evergreen variety.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Molecular and Integrative Physiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Chung, Kyu Hwan

Contributors dc:contributor

• Buetow, Dennis E.
• Korban, Schuyler S.

• Biology, Molecular
• Biology, Botany
• Biology, Animal Physiology

Statement dc:rights

• Copyright 1994 Chung, Kyu Hwan

Language dc:language

eng

Identifier

AAI9416348
(UMI)AAI9416348

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/22623