{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/22623"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/22623","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Isolation, characterization and expression of a peach nuclear gene encoding a light-harvesting chlorophyll a/b-binding polypeptide of photosystem II","abstract":"A nuclear gene encoding a light-harvesting chlorophyll a/b-binding polypeptide of photosystem II has been isolated from a peach genomic library (Prunus persica L. cv. Stark Earliglo) and characterized. The genomic library was constructed and screened with a ($\\alpha$-$\\sp{32}$P) -labeled pea Lhcb1*1 cDNA probe for positive phage containing Lhcb gene sequences. A phage with an Sstl-restricted peach DNA fragment containing an Lhcb gene sequence was identified. This DNA fragment was subcloned (pAB19) and sequenced completely in both directions. pAB19 contains a complete Lhcb gene coding sequence, designated Lhcb1*Pp1, as well as 5$\\sp\\prime$ and 3$\\sp\\prime$ flanking regions. Lhcb1*Pp1 encodes a precursor polypeptide of 267 amino acids composed of a transit peptide of 34 amino acids and a mature polypeptide of 233 amino acids. Transcription initiation and termination sites of Lhcb1*Pp1 mRNA were determined by S1 nuclease mapping. The cap site is located 70 bp upstream from the translation start site and 44 bp downstream from a putative TATA box. Multiple polyadenylation sites are present in the 3$\\sp\\prime$ flanking region. The seasonal expression of Lhcb1*Pp1 was determined by Northern blot and S1 nuclease protection analyses. Both analyses indicate that the relative level of Lhcb1*Pp1 mRNA per unit total leaf RNA declined to a small extent (27%) from the beginning (June) to the end (September) of the growing season, but decreased by 72% with senescence in October. Also, S1 nuclease protection analyses with 5$\\sp\\prime$ and 3$\\sp\\prime$ gene probes showed that Lhcb1*Pp1 is expressed in three varieties of P. persica and in light-grown but its expression is undetectable in dark-grown leaves and stems of the Evergreen variety.","abstract_html":"A nuclear gene encoding a light-harvesting chlorophyll a/b-binding polypeptide of photosystem II has been isolated from a peach genomic library (Prunus persica L. cv. Stark Earliglo) and characterized. The genomic library was constructed and screened with a (<span class=\"etd-inline-math\">&alpha;</span>-$\\sp{32}$P) -labeled pea Lhcb1*1 cDNA probe for positive phage containing Lhcb gene sequences. A phage with an Sstl-restricted peach DNA fragment containing an Lhcb gene sequence was identified. This DNA fragment was subcloned (pAB19) and sequenced completely in both directions. pAB19 contains a complete Lhcb gene coding sequence, designated Lhcb1*Pp1, as well as 5$\\sp\\prime$ and 3$\\sp\\prime$ flanking regions. Lhcb1*Pp1 encodes a precursor polypeptide of 267 amino acids composed of a transit peptide of 34 amino acids and a mature polypeptide of 233 amino acids. Transcription initiation and termination sites of Lhcb1*Pp1 mRNA were determined by S1 nuclease mapping. The cap site is located 70 bp upstream from the translation start site and 44 bp downstream from a putative TATA box. Multiple polyadenylation sites are present in the 3$\\sp\\prime$ flanking region. The seasonal expression of Lhcb1*Pp1 was determined by Northern blot and S1 nuclease protection analyses. Both analyses indicate that the relative level of Lhcb1*Pp1 mRNA per unit total leaf RNA declined to a small extent (27%) from the beginning (June) to the end (September) of the growing season, but decreased by 72% with senescence in October. Also, S1 nuclease protection analyses with 5$\\sp\\prime$ and 3$\\sp\\prime$ gene probes showed that Lhcb1*Pp1 is expressed in three varieties of P. persica and in light-grown but its expression is undetectable in dark-grown leaves and stems of the Evergreen variety.","abstract_has_math":true,"creators":["Chung, Kyu Hwan"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Molecular and Integrative Physiology","degree_department":null,"school":null,"contributors":["Buetow, Dennis E.","Korban, Schuyler S."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T13:45:51Z","date_published":"2011-05-07T13:45:51Z","updated_at":"2026-07-22T22:25:20Z","subjects":["Biology, Molecular","Biology, Botany","Biology, Animal Physiology"],"languages":["eng"],"rights":["Copyright 1994 Chung, Kyu Hwan"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9416348","(UMI)AAI9416348"],"render_values":[{"text":"AAI9416348","href":null,"code":true},{"text":"(UMI)AAI9416348","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/22623","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Buetow, Dennis E.","Korban, Schuyler S."]},{"key":"dc:creator","label":"Author","values":["Chung, Kyu Hwan"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T13:45:51Z","10000-01-01","1994"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Molecular and Integrative Physiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Molecular","Biology, Botany","Biology, Animal Physiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1994 Chung, Kyu Hwan"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9416348","(UMI)AAI9416348","http://hdl.handle.net/2142/22623"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["A nuclear gene encoding a light-harvesting chlorophyll a/b-binding polypeptide of photosystem II has been isolated from a peach genomic library (Prunus persica L. cv. Stark Earliglo) and characterized. The genomic library was constructed and screened with a ($\\alpha$-$\\sp{32}$P) -labeled pea Lhcb1*1 cDNA probe for positive phage containing Lhcb gene sequences. A phage with an Sstl-restricted peach DNA fragment containing an Lhcb gene sequence was identified. This DNA fragment was subcloned (pAB19) and sequenced completely in both directions. pAB19 contains a complete Lhcb gene coding sequence, designated Lhcb1*Pp1, as well as 5$\\sp\\prime$ and 3$\\sp\\prime$ flanking regions. Lhcb1*Pp1 encodes a precursor polypeptide of 267 amino acids composed of a transit peptide of 34 amino acids and a mature polypeptide of 233 amino acids. Transcription initiation and termination sites of Lhcb1*Pp1 mRNA were determined by S1 nuclease mapping. The cap site is located 70 bp upstream from the translation start site and 44 bp downstream from a putative TATA box. Multiple polyadenylation sites are present in the 3$\\sp\\prime$ flanking region. The seasonal expression of Lhcb1*Pp1 was determined by Northern blot and S1 nuclease protection analyses. Both analyses indicate that the relative level of Lhcb1*Pp1 mRNA per unit total leaf RNA declined to a small extent (27%) from the beginning (June) to the end (September) of the growing season, but decreased by 72% with senescence in October. Also, S1 nuclease protection analyses with 5$\\sp\\prime$ and 3$\\sp\\prime$ gene probes showed that Lhcb1*Pp1 is expressed in three varieties of P. persica and in light-grown but its expression is undetectable in dark-grown leaves and stems of the Evergreen variety.","Made available in DSpace on 2011-05-07T13:45:51Z (GMT). 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Stark Earliglo) and characterized. The genomic library was constructed and screened with a ($\\alpha$-$\\sp{32}$P) -labeled pea Lhcb1*1 cDNA probe for positive phage containing Lhcb gene sequences. A phage with an Sstl-restricted peach DNA fragment containing an Lhcb gene sequence was identified. This DNA fragment was subcloned (pAB19) and sequenced completely in both directions. pAB19 contains a complete Lhcb gene coding sequence, designated Lhcb1*Pp1, as well as 5$\\sp\\prime$ and 3$\\sp\\prime$ flanking regions. Lhcb1*Pp1 encodes a precursor polypeptide of 267 amino acids composed of a transit peptide of 34 amino acids and a mature polypeptide of 233 amino acids. Transcription initiation and termination sites of Lhcb1*Pp1 mRNA were determined by S1 nuclease mapping. The cap site is located 70 bp upstream from the translation start site and 44 bp downstream from a putative TATA box. Multiple polyadenylation sites are present in the 3$\\sp\\prime$ flanking region. The seasonal expression of Lhcb1*Pp1 was determined by Northern blot and S1 nuclease protection analyses. Both analyses indicate that the relative level of Lhcb1*Pp1 mRNA per unit total leaf RNA declined to a small extent (27%) from the beginning (June) to the end (September) of the growing season, but decreased by 72% with senescence in October. Also, S1 nuclease protection analyses with 5$\\sp\\prime$ and 3$\\sp\\prime$ gene probes showed that Lhcb1*Pp1 is expressed in three varieties of P. persica and in light-grown but its expression is undetectable in dark-grown leaves and stems of the Evergreen variety.","Made available in DSpace on 2011-05-07T13:45:51Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9416348.pdf: 4478649 bytes, checksum: 2030a50aee0ba2749ad5e7b42927eb17 (MD5) Previous issue date: 1994","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:58:53Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:27:43-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9416348","(UMI)AAI9416348","http://hdl.handle.net/2142/22623"],"dc:language":["eng"],"dc:rights":["Copyright 1994 Chung, Kyu Hwan"],"dc:subject":["Biology, Molecular","Biology, Botany","Biology, Animal Physiology"],"dc:title":["Isolation, characterization and expression of a peach nuclear gene encoding a light-harvesting chlorophyll a/b-binding polypeptide of photosystem II"],"dc:type":["text"],"thesis:degree_discipline":["Molecular and Integrative Physiology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:20Z"}