Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Transit peptide"”.
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Structure and expression of nuclear genes encoding light-harvesting chlorophyll a/b-binding polypeptides of photosystem II in apple and soybean
… for a precursor of 269 amino acids divided by a transit peptide of 40 amino acids and a mature polypeptide of 229 amino acids. This gene also contains a TTGTTT-like polyadenylation signal, and a 5$\sp\prime$-promoter region including TATA-like and CACAT-like boxes. Comparisons between AB10 and …
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Structure and expression of Spinacea oleracea L. and Arabidopsis thaliana complementary-DNAs encoding ribulosebisphosphate carboxylase/oxygenase activase
… consist of two immunologically related polypeptides in the range of 40- to 47-kDa in most plant species examined. Rubisco activase precursor polypeptides are synthesized on cytoplasmic ribosomes and post-translationally imported into the chloroplast with cleavage of a chloroplast transit …
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Characterization of ESl47, a salt stress regulated protein kinase from Lophopyrum elongatum
… of the protein. This region serves as a transit peptide that is required for import of precursor proteins into the chloroplast and is later cleaved. The full length OEE1 clones from Triticum aestivum and Arabidopsis were subcloned into the yeast two-hybrid library vector to determine …
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Isolation, characterization and expression of a peach nuclear gene encoding a light-harvesting chlorophyll a/b-binding polypeptide of photosystem II
… a light-harvesting chlorophyll a/b-binding polypeptide of photosystem II has been isolated from a peach genomic library (Prunus persica L. cv. Stark Earliglo) and characterized. The genomic library was constructed and screened with a ($\alpha$-$\sp{32}$P) -labeled pea Lhcb1*1 cDNA probe for …
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Structure-function relationships for the small subunit (S) of ribulose-1,5-bisphosphate carboxylase/oxygenase: Foreign S expression and characterization of engineered protein
… cassettes targeting the 5'UTR and transit peptide of endogenous Arabidopsis RbcS transcripts. These doubly transformed plants were grown on media with 3% sucrose to cause metabolite repression which can further reduce endogenous Arabidopsis RbcS expression. However, both antisense …
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Study of starch debranching enzymes in developing wheat kernels
… isoamylase pre-protein containing the N-terminal transit peptide of 5 kDa, was isolated from a developing wheat kernel cDNA library. The recombinant protein produced in E. coli by expression of the cDNA exhibited isoamylase-type debranching enzyme activity. Accumulation of the 2.6-kb isoamylase …
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Investigating factors that influence microalgae production platforms for diterpenoids in laboratory and outdoor conditions
… HSP70/RBCS2 promoter and includes a chloroplast transit peptide, and PM51, which in addition includes a thiamine dependent riboswitch (CrTHI4_4N). Casbene-producing *C. reinhardtii* strains were characterised under standard laboratory conditions of 25ºC and 90 μmol s<sup>-1</sup> m<sup>-2</sup> …
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Gene Expression Control for Synthetic Patterning of Bacterial Populations and Plants
… nuclear localization signals and chloroplast transit peptides. For spatiotemporal control of bacteriophage T7 RNA polymerase in M. polymorpha, I characterized spatially restricted and inducible promoters. For facilitated posttranscriptional processing of target transcripts, I functionally …