Modulation of macrophage tumor necrosis factor-alpha production by omega-3 polyunsaturated fatty acids, eicosanoids, and calcium

This research was undertaken to investigate the suppression of tumor necrosis factor-α (TNF-α) production imparted by ω-3 fatty acid (FA) enhancement on macrophage (m$\phi).$ In an in vitro system, murine monocytic cell lines and peritoneal m$\phi$ were incubated with either eicosapentaenoate-BSA (EPA, ω-3), arachidonate-BSA (ARA, ω-6), palmitoleate-BSA (PMOA, ω-7), or BSA. EPA consistently caused $>$60% suppression of LPS-induced TNF-α synthesis. PMOA and BSA had no effect. Recombinant murine interferon-γ (rMulFN-γ) enhanced lipopolysaccharide (LPS)-induced TNF-α production and dose-dependently reduced the suppression caused by EPA. Indomethacin augmented TNF-α production; however, it did not completely abrogate the EPA-induced suppression. ARA caused suppression similar to EPA, but this was totally abrogated by indomethacin. The Ca$\sp{2+}$ ionophore ionomycin was effective as a priming agent and abrogated the EPA-induced suppression when used with LPS.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Food Science and Human Nutrition

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Chen, Steven Enchi

Contributors dc:contributor

• Erdman, John W.

• Agriculture, Food Science and Technology
• Health Sciences, Immunology

Statement dc:rights

• Copyright 1993 Chen, Steven Enchi

Language dc:language

eng

Identifier

AAI9411584
(UMI)AAI9411584

OAI record oai:identifier

https://www.ideals.illinois.edu/oai-pmh?verb=GetRecord&metadataPrefix=etdms&identifier=oai%3Awww.ideals.illinois.edu%3A2142%2F21361