{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/21361"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/21361","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Modulation of macrophage tumor necrosis factor-alpha production by omega-3 polyunsaturated fatty acids, eicosanoids, and calcium","abstract":"Made available in DSpace on 2011-05-07T13:06:27Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9411584.pdf: 5136937 bytes, checksum: e2d5ecc84f036f49d27c51932622b5b8 (MD5) Previous issue date: 1993","abstract_html":"Made available in DSpace on 2011-05-07T13:06:27Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9411584.pdf: 5136937 bytes, checksum: e2d5ecc84f036f49d27c51932622b5b8 (MD5) Previous issue date: 1993","abstract_has_math":false,"creators":["Chen, Steven Enchi"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Food Science and Human Nutrition","degree_department":null,"school":null,"contributors":["Erdman, John W."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T13:06:27Z","date_published":"2011-05-07T13:06:27Z","updated_at":"2026-07-22T22:25:17Z","subjects":["Agriculture, Food Science and Technology","Health Sciences, Immunology"],"languages":["eng"],"rights":["Copyright 1993 Chen, Steven Enchi"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9411584","(UMI)AAI9411584"],"render_values":[{"text":"AAI9411584","href":null,"code":true},{"text":"(UMI)AAI9411584","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/21361","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Erdman, John W."]},{"key":"dc:creator","label":"Author","values":["Chen, Steven Enchi"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T13:06:27Z","10000-01-01","1993"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Food Science and Human Nutrition"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Agriculture, Food Science and Technology","Health Sciences, Immunology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1993 Chen, Steven Enchi"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9411584","(UMI)AAI9411584","http://hdl.handle.net/2142/21361"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Made available in DSpace on 2011-05-07T13:06:27Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9411584.pdf: 5136937 bytes, checksum: e2d5ecc84f036f49d27c51932622b5b8 (MD5) Previous issue date: 1993","This research was undertaken to investigate the suppression of tumor necrosis factor-$\\alpha$ (TNF-$\\alpha)$ production imparted by $\\omega$-3 fatty acid (FA) enhancement on macrophage (m$\\phi).$ In an in vitro system, murine monocytic cell lines and peritoneal m$\\phi$ were incubated with either eicosapentaenoate-BSA (EPA, $\\omega$-3), arachidonate-BSA (ARA, $\\omega$-6), palmitoleate-BSA (PMOA, $\\omega$-7), or BSA. EPA consistently caused $>$60% suppression of LPS-induced TNF-$\\alpha$ synthesis. PMOA and BSA had no effect. Recombinant murine interferon-$\\gamma$ (rMulFN-$\\gamma)$ enhanced lipopolysaccharide (LPS)-induced TNF-$\\alpha$ production and dose-dependently reduced the suppression caused by EPA. Indomethacin augmented TNF-$\\alpha$ production; however, it did not completely abrogate the EPA-induced suppression. ARA caused suppression similar to EPA, but this was totally abrogated by indomethacin. The Ca$\\sp{2+}$ ionophore ionomycin was effective as a priming agent and abrogated the EPA-induced suppression when used with LPS.","Eicosanoids derived from ARA (PGE$\\sb2)$ and EPA (PGE$\\sb3)$ are structurally different. Both PGE$\\sb2$ and PGE$\\sb3$ dose-dependently suppressed TNF-$\\alpha.$ Their efficacy was dependent on concentration and the level of m$\\phi$ stimulation, in the absence or presence of indomethacin.","TNF-$\\alpha$ mRNA levels of control and EPA-enhanced groups were compared. When IFN-$\\gamma$ was used, the mRNA levels of EPA-treated groups were equal in magnitude to the control groups'. Therefore, the observed suppression at the cellular level could not be attributed to differential levels of mRNA accumulation at low activational states. When ionomycin was used as a priming agent, the mRNA levels for the EPA group was consistently higher than that of the controls', which, at the cellular level, was paralleled with abrogation of the differences in TNF-$\\alpha$ detected between groups. Together, the findings suggested that post-transcriptional processes were responsible for the observed suppression of TNF-$\\alpha.$","From these findings, $\\omega$-3 FA enhancement of murine m$\\phi$-induced suppression of TNF-$\\alpha$ by a minimum of two paths. First, PGE$\\sb3$ derived from EPA could directly cause suppression of TNF-$\\alpha.$ Second, ionomycin, IFN-$\\gamma$ and PKc activation demonstrated that up-regulation of this pathway could reverse the effects of $\\omega$-3 FA enhancement.","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:50:15Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:22:56-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Modulation of macrophage tumor necrosis factor-alpha production by omega-3 polyunsaturated fatty acids, eicosanoids, and calcium"]}]}],"canonical_facts":{"dc:contributor":["Erdman, John W."],"dc:creator":["Chen, Steven Enchi"],"dc:date":["2011-05-07T13:06:27Z","10000-01-01","1993"],"dc:description":["Made available in DSpace on 2011-05-07T13:06:27Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9411584.pdf: 5136937 bytes, checksum: e2d5ecc84f036f49d27c51932622b5b8 (MD5) Previous issue date: 1993","This research was undertaken to investigate the suppression of tumor necrosis factor-$\\alpha$ (TNF-$\\alpha)$ production imparted by $\\omega$-3 fatty acid (FA) enhancement on macrophage (m$\\phi).$ In an in vitro system, murine monocytic cell lines and peritoneal m$\\phi$ were incubated with either eicosapentaenoate-BSA (EPA, $\\omega$-3), arachidonate-BSA (ARA, $\\omega$-6), palmitoleate-BSA (PMOA, $\\omega$-7), or BSA. EPA consistently caused $>$60% suppression of LPS-induced TNF-$\\alpha$ synthesis. PMOA and BSA had no effect. Recombinant murine interferon-$\\gamma$ (rMulFN-$\\gamma)$ enhanced lipopolysaccharide (LPS)-induced TNF-$\\alpha$ production and dose-dependently reduced the suppression caused by EPA. Indomethacin augmented TNF-$\\alpha$ production; however, it did not completely abrogate the EPA-induced suppression. ARA caused suppression similar to EPA, but this was totally abrogated by indomethacin. The Ca$\\sp{2+}$ ionophore ionomycin was effective as a priming agent and abrogated the EPA-induced suppression when used with LPS.","Eicosanoids derived from ARA (PGE$\\sb2)$ and EPA (PGE$\\sb3)$ are structurally different. Both PGE$\\sb2$ and PGE$\\sb3$ dose-dependently suppressed TNF-$\\alpha.$ Their efficacy was dependent on concentration and the level of m$\\phi$ stimulation, in the absence or presence of indomethacin.","TNF-$\\alpha$ mRNA levels of control and EPA-enhanced groups were compared. When IFN-$\\gamma$ was used, the mRNA levels of EPA-treated groups were equal in magnitude to the control groups'. Therefore, the observed suppression at the cellular level could not be attributed to differential levels of mRNA accumulation at low activational states. When ionomycin was used as a priming agent, the mRNA levels for the EPA group was consistently higher than that of the controls', which, at the cellular level, was paralleled with abrogation of the differences in TNF-$\\alpha$ detected between groups. Together, the findings suggested that post-transcriptional processes were responsible for the observed suppression of TNF-$\\alpha.$","From these findings, $\\omega$-3 FA enhancement of murine m$\\phi$-induced suppression of TNF-$\\alpha$ by a minimum of two paths. First, PGE$\\sb3$ derived from EPA could directly cause suppression of TNF-$\\alpha.$ Second, ionomycin, IFN-$\\gamma$ and PKc activation demonstrated that up-regulation of this pathway could reverse the effects of $\\omega$-3 FA enhancement.","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:50:15Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:22:56-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9411584","(UMI)AAI9411584","http://hdl.handle.net/2142/21361"],"dc:language":["eng"],"dc:rights":["Copyright 1993 Chen, Steven Enchi"],"dc:subject":["Agriculture, Food Science and Technology","Health Sciences, Immunology"],"dc:title":["Modulation of macrophage tumor necrosis factor-alpha production by omega-3 polyunsaturated fatty acids, eicosanoids, and calcium"],"dc:type":["text"],"thesis:degree_discipline":["Food Science and Human Nutrition"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:17Z"}