Characterization and nucleotide sequence of apple mosaic virus coat protein gene

Apple mosaic virus (ApMV) (Idaho strain) was purified from infected cucumber cotyledons. Virions 3 and 4 (top components in sucrose gradient) were isolated and RNA was extracted. A double stranded copy DNA (cDNA) was synthesized from in vitro polyadenylated RNA3 and cloned into a pBluescript T-vector. The inserts of four clones from the 3$\sp\prime$-end of RNA3 were sequenced and the 3$\sp\prime$-primer was designed to synthesize cDNA of RNA3. The DNA was cloned into the plasmid vector pUC118. A set of overlapping cDNA clones was used to determine the nucleotide sequence of RNA4. The 5$\sp\prime$-end of RNA4 was mapped using the primer extension technique. ApMV RNA4 was found to be 983 nucleotides in length containing open reading frame (ORF) of a 662 nucleotides, which was flanked by 5$\sp\prime$ and 3$\sp\prime$-nontranslated sequence of 55 and 265 nucleotides, respectively. The ORF encodes for a coat protein of a 25,085 Mr. The sequence contains several inverted repeats that could form hair-pin structures. In addition, two palindromic sequence were found in the ORF with a third palindrome in the 3$\sp\prime$-nontranslated region. These structures may be involved in the recognition of RNA by viral replicase or in genome activation by the coat protein. The amino-terminus of ApMV coat protein is basic and rich in Cys, whereas the carboxy-terminus has an even distribution of basic and acidic amino acids, similar to other ilarviruses.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Alrefai, Rudaina Hussein

Contributors dc:contributor

• Korban, Schuyler S.

• Agriculture, Agronomy
• Biology, Molecular
• Agriculture, Plant Pathology

Statement dc:rights

• Copyright 1993 Alrefai, Rudaina Hussein

Language dc:language

eng

Identifier

AAI9314835
(UMI)AAI9314835

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/21141