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Showing 1 to 6 of 6 for “"pBlueScript"”.

  1. Molecular characterisation of the fibre-digesting bacteria isolated from the ostrich (Struthio Camelus var. Domesticus) hindgut

    … cloning the products into the plasmid vector pBluescript (SK). The results obtained from the 16S rDNA sequence homologies strongly suggests that the newly identified bacteria should be assigned to three major bacterial genera, namely, Ruminococcus, Buryrivibrio and Bacteroides. Four highly …

    cape-town Repository record for Molecular characterisation of the fibre-digesting bacteria isolated from the ostrich (Struthio Camelus var. Domesticus) hindgut (opens in a new tab)

  2. Carte de restriction et clonage de certains fragments de l'ADN mitochondrial de la palourde de dune Spisula solidissima

    … Spisula solidissima a été clonée dans le vecteur pBluescript®. La grandeur des fragments clonés était 2.9 kpb, 1.3 kpb et 0.9 kpb. Les résultats de cette recherche permettront l’étude approfondie de la molécule d’ADNmt de taille “ atypique ” de Spisula solidissima et le séquençage éventuel de son …

    moncton Repository record for Carte de restriction et clonage de certains fragments de l'ADN mitochondrial de la palourde de dune Spisula solidissima (opens in a new tab)

  3. Characterization and nucleotide sequence of apple mosaic virus coat protein gene

    … in vitro polyadenylated RNA3 and cloned into a pBluescript T-vector. The inserts of four clones from the 3$\sp\prime$-end of RNA3 were sequenced and the 3$\sp\prime$-primer was designed to synthesize cDNA of RNA3. The DNA was cloned into the plasmid vector pUC118. A set of overlapping cDNA …

    uiuc Repository record for Characterization and nucleotide sequence of apple mosaic virus coat protein gene (opens in a new tab)

  4. Molecular and biochemical characterization of phospholipase D in cotton (Gossypium hirsutum L) seedlings.

    … from the Uni-ZAP lambda library, excised as pBluescript SK(-) phagemids and subjected to nucleotide sequence analysis. Alignment of derived sequences with Arabidopsis PLD family members indicated that the cDNAs represent six different PLD gene products -three putative PLD β isoforms and three …

    unt Repository record for Molecular and biochemical characterization of phospholipase D in cotton (Gossypium hirsutum L) seedlings. (opens in a new tab)

  5. Protein factors and 5' flanking sequences involved in the expression of the mouse myelin basic protein gene.

    … of pEX1 plasmid was subcloned in the vector pBluescript. The cloned insert, which corresponds to the region between nucleotides -1319 and +227 relative to the transcription start site of the mouse MBP gene, was subsequently sequenced manually using the chain termination method. Sequence …

    sheffield-hallam Repository record for Protein factors and 5' flanking sequences involved in the expression of the mouse myelin basic protein gene. (opens in a new tab)

  6. Generation of cDNA libraries of amoeba, 8 hour, and 12 hour stages of Dictyostelium discoideum

    A critical event during the life cycle of Dictyostelium discoideum is glycogen turnover. This process is catalyzed by glycogen phosphorylase-2 (gp-2). Since gp-2 expression is first induced during the transition from growth to differentiation, understanding how this gene is controlled may provide …

    vt Repository record for Generation of cDNA libraries of amoeba, 8 hour, and 12 hour stages of Dictyostelium discoideum (opens in a new tab)