{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/21141"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/21141","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Characterization and nucleotide sequence of apple mosaic virus coat protein gene","abstract":"Apple mosaic virus (ApMV) (Idaho strain) was purified from infected cucumber cotyledons. Virions 3 and 4 (top components in sucrose gradient) were isolated and RNA was extracted. A double stranded copy DNA (cDNA) was synthesized from in vitro polyadenylated RNA3 and cloned into a pBluescript T-vector. The inserts of four clones from the 3$\\sp\\prime$-end of RNA3 were sequenced and the 3$\\sp\\prime$-primer was designed to synthesize cDNA of RNA3. The DNA was cloned into the plasmid vector pUC118. A set of overlapping cDNA clones was used to determine the nucleotide sequence of RNA4. The 5$\\sp\\prime$-end of RNA4 was mapped using the primer extension technique. ApMV RNA4 was found to be 983 nucleotides in length containing open reading frame (ORF) of a 662 nucleotides, which was flanked by 5$\\sp\\prime$ and 3$\\sp\\prime$-nontranslated sequence of 55 and 265 nucleotides, respectively. The ORF encodes for a coat protein of a 25,085 Mr. The sequence contains several inverted repeats that could form hair-pin structures. In addition, two palindromic sequence were found in the ORF with a third palindrome in the 3$\\sp\\prime$-nontranslated region. These structures may be involved in the recognition of RNA by viral replicase or in genome activation by the coat protein. The amino-terminus of ApMV coat protein is basic and rich in Cys, whereas the carboxy-terminus has an even distribution of basic and acidic amino acids, similar to other ilarviruses.","abstract_html":"Apple mosaic virus (ApMV) (Idaho strain) was purified from infected cucumber cotyledons. Virions 3 and 4 (top components in sucrose gradient) were isolated and RNA was extracted. A double stranded copy DNA (cDNA) was synthesized from in vitro polyadenylated RNA3 and cloned into a pBluescript T-vector. The inserts of four clones from the 3$\\sp\\prime$-end of RNA3 were sequenced and the 3$\\sp\\prime$-primer was designed to synthesize cDNA of RNA3. The DNA was cloned into the plasmid vector pUC118. A set of overlapping cDNA clones was used to determine the nucleotide sequence of RNA4. The 5$\\sp\\prime$-end of RNA4 was mapped using the primer extension technique. ApMV RNA4 was found to be 983 nucleotides in length containing open reading frame (ORF) of a 662 nucleotides, which was flanked by 5$\\sp\\prime$ and 3$\\sp\\prime$-nontranslated sequence of 55 and 265 nucleotides, respectively. The ORF encodes for a coat protein of a 25,085 Mr. The sequence contains several inverted repeats that could form hair-pin structures. In addition, two palindromic sequence were found in the ORF with a third palindrome in the 3$\\sp\\prime$-nontranslated region. These structures may be involved in the recognition of RNA by viral replicase or in genome activation by the coat protein. The amino-terminus of ApMV coat protein is basic and rich in Cys, whereas the carboxy-terminus has an even distribution of basic and acidic amino acids, similar to other ilarviruses.","abstract_has_math":true,"creators":["Alrefai, Rudaina Hussein"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Korban, Schuyler S."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T12:59:35Z","date_published":"2011-05-07T12:59:35Z","updated_at":"2026-07-22T22:25:17Z","subjects":["Agriculture, Agronomy","Biology, Molecular","Agriculture, Plant Pathology"],"languages":["eng"],"rights":["Copyright 1993 Alrefai, Rudaina Hussein"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9314835","(UMI)AAI9314835"],"render_values":[{"text":"AAI9314835","href":null,"code":true},{"text":"(UMI)AAI9314835","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/21141","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Korban, Schuyler S."]},{"key":"dc:creator","label":"Author","values":["Alrefai, Rudaina Hussein"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T12:59:35Z","10000-01-01","1993"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Agriculture, Agronomy","Biology, Molecular","Agriculture, Plant Pathology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1993 Alrefai, Rudaina Hussein"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9314835","(UMI)AAI9314835","http://hdl.handle.net/2142/21141"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Apple mosaic virus (ApMV) (Idaho strain) was purified from infected cucumber cotyledons. Virions 3 and 4 (top components in sucrose gradient) were isolated and RNA was extracted. A double stranded copy DNA (cDNA) was synthesized from in vitro polyadenylated RNA3 and cloned into a pBluescript T-vector. The inserts of four clones from the 3$\\sp\\prime$-end of RNA3 were sequenced and the 3$\\sp\\prime$-primer was designed to synthesize cDNA of RNA3. The DNA was cloned into the plasmid vector pUC118. A set of overlapping cDNA clones was used to determine the nucleotide sequence of RNA4. The 5$\\sp\\prime$-end of RNA4 was mapped using the primer extension technique. ApMV RNA4 was found to be 983 nucleotides in length containing open reading frame (ORF) of a 662 nucleotides, which was flanked by 5$\\sp\\prime$ and 3$\\sp\\prime$-nontranslated sequence of 55 and 265 nucleotides, respectively. The ORF encodes for a coat protein of a 25,085 Mr. The sequence contains several inverted repeats that could form hair-pin structures. In addition, two palindromic sequence were found in the ORF with a third palindrome in the 3$\\sp\\prime$-nontranslated region. These structures may be involved in the recognition of RNA by viral replicase or in genome activation by the coat protein. The amino-terminus of ApMV coat protein is basic and rich in Cys, whereas the carboxy-terminus has an even distribution of basic and acidic amino acids, similar to other ilarviruses.","\"To transform plants with ApMV coat protein gene an Agrobacterium-mediated transformation system was developed for apple \"\"Royal Gala\"\" plants. Kanamycin completely inhibited regeneration of untransformed leaf sections on a 1/2 Murashige and Skoog (MS) regeneration medium. Apple leaf sections were transformed by Agrobacterium strains C58Z707:pZA-7 and EHA101:pZA-7 and calli were produced on a 1/2MS selection medium.\"","Made available in DSpace on 2011-05-07T12:59:35Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9314835.pdf: 3055004 bytes, checksum: 6709f0de44347cc4a866c81095f90774 (MD5) Previous issue date: 1993","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:48:46Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:22:07-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Characterization and nucleotide sequence of apple mosaic virus coat protein gene"]}]}],"canonical_facts":{"dc:contributor":["Korban, Schuyler S."],"dc:creator":["Alrefai, Rudaina Hussein"],"dc:date":["2011-05-07T12:59:35Z","10000-01-01","1993"],"dc:description":["Apple mosaic virus (ApMV) (Idaho strain) was purified from infected cucumber cotyledons. Virions 3 and 4 (top components in sucrose gradient) were isolated and RNA was extracted. A double stranded copy DNA (cDNA) was synthesized from in vitro polyadenylated RNA3 and cloned into a pBluescript T-vector. The inserts of four clones from the 3$\\sp\\prime$-end of RNA3 were sequenced and the 3$\\sp\\prime$-primer was designed to synthesize cDNA of RNA3. The DNA was cloned into the plasmid vector pUC118. A set of overlapping cDNA clones was used to determine the nucleotide sequence of RNA4. The 5$\\sp\\prime$-end of RNA4 was mapped using the primer extension technique. ApMV RNA4 was found to be 983 nucleotides in length containing open reading frame (ORF) of a 662 nucleotides, which was flanked by 5$\\sp\\prime$ and 3$\\sp\\prime$-nontranslated sequence of 55 and 265 nucleotides, respectively. The ORF encodes for a coat protein of a 25,085 Mr. The sequence contains several inverted repeats that could form hair-pin structures. In addition, two palindromic sequence were found in the ORF with a third palindrome in the 3$\\sp\\prime$-nontranslated region. These structures may be involved in the recognition of RNA by viral replicase or in genome activation by the coat protein. The amino-terminus of ApMV coat protein is basic and rich in Cys, whereas the carboxy-terminus has an even distribution of basic and acidic amino acids, similar to other ilarviruses.","\"To transform plants with ApMV coat protein gene an Agrobacterium-mediated transformation system was developed for apple \"\"Royal Gala\"\" plants. Kanamycin completely inhibited regeneration of untransformed leaf sections on a 1/2 Murashige and Skoog (MS) regeneration medium. Apple leaf sections were transformed by Agrobacterium strains C58Z707:pZA-7 and EHA101:pZA-7 and calli were produced on a 1/2MS selection medium.\"","Made available in DSpace on 2011-05-07T12:59:35Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9314835.pdf: 3055004 bytes, checksum: 6709f0de44347cc4a866c81095f90774 (MD5) Previous issue date: 1993","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:48:46Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:22:07-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9314835","(UMI)AAI9314835","http://hdl.handle.net/2142/21141"],"dc:language":["eng"],"dc:rights":["Copyright 1993 Alrefai, Rudaina Hussein"],"dc:subject":["Agriculture, Agronomy","Biology, Molecular","Agriculture, Plant Pathology"],"dc:title":["Characterization and nucleotide sequence of apple mosaic virus coat protein gene"],"dc:type":["text"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:17Z"}