Nanoparticle intervention for control of cell migration

Nanoparticles exhibit remarkable physiochemical properties unique from their bulk material counterparts. Nanoparticles, and gold nanoparticles in particular, have drawn much interest for use in biomedical applications. Much of the work in the area of biological-nanoparticle interactions focuses on how changes in nanoparticle properties affect cellular uptake, cytotoxicity, and gene expression. However, it has been shown that nanoparticles can have an indirect impact on cells by changing the local microenvironment, causing a change in cell behavior. It is essential to understand the full spectrum of nanoparticle effects on biological systems to ensure safe future therapeutics. In this work, we will first discuss the basics of AuNP synthesis and the biological applications of AuNPs in Chapter 1; research on the nano-bio interface is detailed, and we explore how nanoparticles can influence biological behavior through indirect interactions with the cellular microenvironment. Chemotaxis and cell migration are discussed as a model system for studying indirect nano-bio interactions. In Chapter 2, we analyze how tuning the surface chemistry of AuNPs to have varying levels of sulfonation affects the adsorption of monocyte chemoattractant protein 1 and downstream disruption of human monocytic cell line THP-1 chemotaxis. Chapter 3 discusses our attempt to deconvolute the chemical and mechanical signaling modalities that control colorectal cancer cell HCT-116 migration. Specifically, we attempt to characterize HCT-116 migratory response to chemical and mechanical modulation of the extracellular matrix by cancer associated fibroblasts.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2024

Author dc:creator

• Tetrick, Maxwell G.

Contributors dc:contributor

• Murphy, Catherine
• Chan, Jefferson
• Rodriguez-Lopez, Joaquin
• Sweedler, Jonathan

• Gold Nanoparticles
• Cell Migration
• Surface Chemistry
• Chemotaxis

Statement dc:rights

• Copyright 2024 Maxwell Tetrick

Language dc:language

en, eng

Handle dc:identifier

https://hdl.handle.net/2142/125762

OAI record oai:identifier

https://www.ideals.illinois.edu/oai-pmh?verb=GetRecord&metadataPrefix=etdms&identifier=oai%3Awww.ideals.illinois.edu%3A2142%2F125762