Immune responses alter in heterogeneous nuclear ribonucleoprotein I knockout mice with medium protein diet or probiotic intervention

Colon cancer is the third most common cancer in both men and women. Diet and gut microbiome play critical roles in regulating the incidence of colon cancer. The effects of dietary protein in colon cancer remain unclear. The main goal of this present study is to identify whether the medium protein diet reduces inflammatory responses and colon cancer risks by regulating IgA production. In recent years, more studies found that probiotics have advantages on prevent colon cancer. The other objective of the study was to test whether Bifidobacterium gavage reduces inflammations in the colon by changing IgA plasma cell production. In the first part of the thesis study, wildtype and hnRNPI knockout mice were fed with either control AIN-93 diet (14.41 % kcal from protein) or a medium protein diet (28.83% kcal from protein) for 25-35 weeks. And we measured the immune cells and physiological phenotypes. The data indicated that medium protein reduced intestinal inflammation in female hnRNPI knockout mice only. IgA production in T cell-independent manner might be one of the reasons that cause this result. And T cell-dependent IgA plasma cell number of female mice is not affected by the protein level difference between diets. In the second part of the study, both wildtype and hnRNPI knockout mice at age 12-16-week-old were fed with a control AIN-93 diet and received 1×109 CFU/ day Bifidobacterium gavage treatment for 10 weeks. We measured the colon length and immune cells. The data indicated that Bifidobacterium treatment reduced the inflammatory response only in female hnRNPI knockout mice, and T cell-independent IgA plasma cells might contribute to the effect. Conclusively, the medium protein diet and oral probiotic intake might reduce the inflammation in the colon of female hnRNPI knockout mice, and T cell-independent IgA plasma cell production might be one of the reasons that induce this outcome. More experiments on other plasma cells and gene expression are necessary for future study.

Name thesis:degree_name

M.S.

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Nutritional Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2021

Author dc:creator

• Zhao, Yingwei

Contributors dc:contributor

• Pan, Yuan-Xiang
• Chen, Hong
• Arthur, Anna E

• Nutrition
• Medium Protein
• Diet
• Probiotic
• Colon Cancer
• IgA

Statement dc:rights

• Copyright 2021 Yingwei Zhao

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/110868

OAI record oai:identifier

https://www.ideals.illinois.edu/oai-pmh?verb=GetRecord&metadataPrefix=etdms&identifier=oai%3Awww.ideals.illinois.edu%3A2142%2F110868