{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/110868"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/110868","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Immune responses alter in heterogeneous nuclear ribonucleoprotein I knockout mice with medium protein diet or probiotic intervention","abstract":"The student, Yingwei Zhao, submitted this Thesis for approval on 2021-04-27 at 14:06.","abstract_html":"The student, Yingwei Zhao, submitted this Thesis for approval on 2021-04-27 at 14:06.","abstract_has_math":false,"creators":["Zhao, Yingwei"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Nutritional Sciences","degree_department":null,"school":null,"contributors":["Pan, Yuan-Xiang","Chen, Hong","Arthur, Anna E"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2021,"date_issued":"2021-09-17T04:06:56Z","date_published":"2021-09-17T04:06:56Z","updated_at":"2026-07-22T22:24:52Z","subjects":["Nutrition","Medium Protein","Diet","Probiotic","Colon Cancer","IgA"],"languages":["en"],"rights":["Copyright 2021 Yingwei Zhao"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/110868","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Pan, Yuan-Xiang","Chen, Hong","Arthur, Anna E"]},{"key":"dc:creator","label":"Author","values":["Zhao, Yingwei"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2021-09-17T04:06:56Z","2023-09-17T04:07:01Z","2021-04-28","2021-05"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Nutritional Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Nutrition","Medium Protein","Diet","Probiotic","Colon Cancer","IgA"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2021 Yingwei Zhao"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/110868"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The student, Yingwei Zhao, submitted this Thesis for approval on 2021-04-27 at 14:06.","This Thesis was approved for publication on 2021-04-28 at 14:48.","DSpace SAF Submission Ingestion Package generated from Vireo submission #16586 on 2021-09-16 at 20:14:38","Colon cancer is the third most common cancer in both men and women. Diet and gut microbiome play critical roles in regulating the incidence of colon cancer. The effects of dietary protein in colon cancer remain unclear. The main goal of this present study is to identify whether the medium protein diet reduces inflammatory responses and colon cancer risks by regulating IgA production. In recent years, more studies found that probiotics have advantages on prevent colon cancer. The other objective of the study was to test whether Bifidobacterium gavage reduces inflammations in the colon by changing IgA plasma cell production. In the first part of the thesis study, wildtype and hnRNPI knockout mice were fed with either control AIN-93 diet (14.41 % kcal from protein) or a medium protein diet (28.83% kcal from protein) for 25-35 weeks. And we measured the immune cells and physiological phenotypes. The data indicated that medium protein reduced intestinal inflammation in female hnRNPI knockout mice only. IgA production in T cell-independent manner might be one of the reasons that cause this result. And T cell-dependent IgA plasma cell number of female mice is not affected by the protein level difference between diets. In the second part of the study, both wildtype and hnRNPI knockout mice at age 12-16-week-old were fed with a control AIN-93 diet and received 1×109 CFU/ day Bifidobacterium gavage treatment for 10 weeks. We measured the colon length and immune cells. The data indicated that Bifidobacterium treatment reduced the inflammatory response only in female hnRNPI knockout mice, and T cell-independent IgA plasma cells might contribute to the effect. Conclusively, the medium protein diet and oral probiotic intake might reduce the inflammation in the colon of female hnRNPI knockout mice, and T cell-independent IgA plasma cell production might be one of the reasons that induce this outcome. More experiments on other plasma cells and gene expression are necessary for future study.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2023-05-01","The student, Yingwei Zhao, accepted the attached license on 2021-04-27 at 13:32.","Made available in DSpace on 2021-09-17T04:06:56Z (GMT). No. of bitstreams: 2 ZHAO-THESIS-2021.pdf: 1970540 bytes, checksum: 4bc48801e2c95d6329565f335a761272 (MD5) LICENSE.txt: 4209 bytes, checksum: efdb39338d4c9b71b507fd771d0ac0e8 (MD5) Previous issue date: 2021-04-28","Embargo set by: Seth Robbins for item 118714 Lift date: 2023-09-17T04:07:01Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Immune responses alter in heterogeneous nuclear ribonucleoprotein I knockout mice with medium protein diet or probiotic intervention"]}]}],"canonical_facts":{"dc:contributor":["Pan, Yuan-Xiang","Chen, Hong","Arthur, Anna E"],"dc:creator":["Zhao, Yingwei"],"dc:date":["2021-09-17T04:06:56Z","2023-09-17T04:07:01Z","2021-04-28","2021-05"],"dc:description":["The student, Yingwei Zhao, submitted this Thesis for approval on 2021-04-27 at 14:06.","This Thesis was approved for publication on 2021-04-28 at 14:48.","DSpace SAF Submission Ingestion Package generated from Vireo submission #16586 on 2021-09-16 at 20:14:38","Colon cancer is the third most common cancer in both men and women. Diet and gut microbiome play critical roles in regulating the incidence of colon cancer. The effects of dietary protein in colon cancer remain unclear. The main goal of this present study is to identify whether the medium protein diet reduces inflammatory responses and colon cancer risks by regulating IgA production. In recent years, more studies found that probiotics have advantages on prevent colon cancer. The other objective of the study was to test whether Bifidobacterium gavage reduces inflammations in the colon by changing IgA plasma cell production. In the first part of the thesis study, wildtype and hnRNPI knockout mice were fed with either control AIN-93 diet (14.41 % kcal from protein) or a medium protein diet (28.83% kcal from protein) for 25-35 weeks. And we measured the immune cells and physiological phenotypes. The data indicated that medium protein reduced intestinal inflammation in female hnRNPI knockout mice only. IgA production in T cell-independent manner might be one of the reasons that cause this result. And T cell-dependent IgA plasma cell number of female mice is not affected by the protein level difference between diets. In the second part of the study, both wildtype and hnRNPI knockout mice at age 12-16-week-old were fed with a control AIN-93 diet and received 1×109 CFU/ day Bifidobacterium gavage treatment for 10 weeks. We measured the colon length and immune cells. The data indicated that Bifidobacterium treatment reduced the inflammatory response only in female hnRNPI knockout mice, and T cell-independent IgA plasma cells might contribute to the effect. Conclusively, the medium protein diet and oral probiotic intake might reduce the inflammation in the colon of female hnRNPI knockout mice, and T cell-independent IgA plasma cell production might be one of the reasons that induce this outcome. More experiments on other plasma cells and gene expression are necessary for future study.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2023-05-01","The student, Yingwei Zhao, accepted the attached license on 2021-04-27 at 13:32.","Made available in DSpace on 2021-09-17T04:06:56Z (GMT). No. of bitstreams: 2 ZHAO-THESIS-2021.pdf: 1970540 bytes, checksum: 4bc48801e2c95d6329565f335a761272 (MD5) LICENSE.txt: 4209 bytes, checksum: efdb39338d4c9b71b507fd771d0ac0e8 (MD5) Previous issue date: 2021-04-28","Embargo set by: Seth Robbins for item 118714 Lift date: 2023-09-17T04:07:01Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/110868"],"dc:language":["en"],"dc:rights":["Copyright 2021 Yingwei Zhao"],"dc:subject":["Nutrition","Medium Protein","Diet","Probiotic","Colon Cancer","IgA"],"dc:title":["Immune responses alter in heterogeneous nuclear ribonucleoprotein I knockout mice with medium protein diet or probiotic intervention"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Nutritional Sciences"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:52Z"}