Protein interactions in vitro and in the cell

The cell is a complex crowded environment containing up to 300 mg/mL bio-macromolecules. In this environment proteins are far from isolated systems and interact with many other biological molecules they encounter. In recent years, better instrumentation and greater computing power have enabled imaging of elusive biomolecule dynamics in cells, driving many advances in understanding the chemical organization of biological systems. Interactions in the cell affect both biomolecular stability and function. The same protein or nucleic acid can behave differently depending on time in the cell cycle, location in a specific compartment, or stresses acting on the cell. My thesis focuses on these interactions both in vitro and in the cell and the differences between a model in vitro environment and the cell. The broader goal of my research is to emphasize the importance of the cellular environment in designing future biological studies.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2020

Author dc:creator

• Guin, Drishti

Contributors dc:contributor

• Gruebele, Martin
• Leckband, Deborah
• Freeman, Brian
• Shukla, Diwakar

• Protein binding, in cell, in vitro, dodine, denaturant, protein interactions

Statement dc:rights

• Copyright 2019 Drishti Guin

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/106142

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/106142