{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/106142"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/106142","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Protein interactions in vitro and in the cell","abstract":"The cell is a complex crowded environment containing up to 300 mg/mL bio-macromolecules. In this environment proteins are far from isolated systems and interact with many other biological molecules they encounter. In recent years, better instrumentation and greater computing power have enabled imaging of elusive biomolecule dynamics in cells, driving many advances in understanding the chemical organization of biological systems. Interactions in the cell affect both biomolecular stability and function. The same protein or nucleic acid can behave differently depending on time in the cell cycle, location in a specific compartment, or stresses acting on the cell. My thesis focuses on these interactions both in vitro and in the cell and the differences between a model in vitro environment and the cell. The broader goal of my research is to emphasize the importance of the cellular environment in designing future biological studies.","abstract_html":"The cell is a complex crowded environment containing up to 300 mg/mL bio-macromolecules. In this environment proteins are far from isolated systems and interact with many other biological molecules they encounter. In recent years, better instrumentation and greater computing power have enabled imaging of elusive biomolecule dynamics in cells, driving many advances in understanding the chemical organization of biological systems. Interactions in the cell affect both biomolecular stability and function. The same protein or nucleic acid can behave differently depending on time in the cell cycle, location in a specific compartment, or stresses acting on the cell. My thesis focuses on these interactions both in vitro and in the cell and the differences between a model in vitro environment and the cell. The broader goal of my research is to emphasize the importance of the cellular environment in designing future biological studies.","abstract_has_math":false,"creators":["Guin, Drishti"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Gruebele, Martin","Leckband, Deborah","Freeman, Brian","Shukla, Diwakar"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2020,"date_issued":"2020-03-02T21:57:54Z","date_published":"2020-03-02T21:57:54Z","updated_at":"2026-07-22T22:24:45Z","subjects":["Protein binding, in cell, in vitro, dodine, denaturant, protein interactions"],"languages":["en"],"rights":["Copyright 2019 Drishti Guin"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/106142","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Gruebele, Martin","Leckband, Deborah","Freeman, Brian","Shukla, Diwakar"]},{"key":"dc:creator","label":"Author","values":["Guin, Drishti"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2020-03-02T21:57:54Z","2019-08-23","2019-12"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Protein binding, in cell, in vitro, dodine, denaturant, protein interactions"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2019 Drishti Guin"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/106142"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The cell is a complex crowded environment containing up to 300 mg/mL bio-macromolecules. In this environment proteins are far from isolated systems and interact with many other biological molecules they encounter. In recent years, better instrumentation and greater computing power have enabled imaging of elusive biomolecule dynamics in cells, driving many advances in understanding the chemical organization of biological systems. Interactions in the cell affect both biomolecular stability and function. The same protein or nucleic acid can behave differently depending on time in the cell cycle, location in a specific compartment, or stresses acting on the cell. My thesis focuses on these interactions both in vitro and in the cell and the differences between a model in vitro environment and the cell. The broader goal of my research is to emphasize the importance of the cellular environment in designing future biological studies.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2020-02-28 without embargo terms","The student, Drishti Guin, accepted the attached license on 2019-08-13 at 18:23.","The student, Drishti Guin, submitted this Dissertation for approval on 2019-08-13 at 18:29.","This Dissertation was approved for publication on 2019-08-23 at 09:30.","DSpace SAF Submission Ingestion Package generated from Vireo submission #14421 on 2020-02-28 at 17:11:09","Made available in DSpace on 2020-03-02T21:57:54Z (GMT). No. of bitstreams: 2 GUIN-DISSERTATION-2019.pdf: 12101441 bytes, checksum: 3c4224d3e7b92270e415798a9995f463 (MD5) LICENSE.txt: 4209 bytes, checksum: b81203a711fa166c28e2eb01d269aef1 (MD5) Previous issue date: 2019-08-23"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Protein interactions in vitro and in the cell"]}]}],"canonical_facts":{"dc:contributor":["Gruebele, Martin","Leckband, Deborah","Freeman, Brian","Shukla, Diwakar"],"dc:creator":["Guin, Drishti"],"dc:date":["2020-03-02T21:57:54Z","2019-08-23","2019-12"],"dc:description":["The cell is a complex crowded environment containing up to 300 mg/mL bio-macromolecules. In this environment proteins are far from isolated systems and interact with many other biological molecules they encounter. In recent years, better instrumentation and greater computing power have enabled imaging of elusive biomolecule dynamics in cells, driving many advances in understanding the chemical organization of biological systems. Interactions in the cell affect both biomolecular stability and function. The same protein or nucleic acid can behave differently depending on time in the cell cycle, location in a specific compartment, or stresses acting on the cell. My thesis focuses on these interactions both in vitro and in the cell and the differences between a model in vitro environment and the cell. The broader goal of my research is to emphasize the importance of the cellular environment in designing future biological studies.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2020-02-28 without embargo terms","The student, Drishti Guin, accepted the attached license on 2019-08-13 at 18:23.","The student, Drishti Guin, submitted this Dissertation for approval on 2019-08-13 at 18:29.","This Dissertation was approved for publication on 2019-08-23 at 09:30.","DSpace SAF Submission Ingestion Package generated from Vireo submission #14421 on 2020-02-28 at 17:11:09","Made available in DSpace on 2020-03-02T21:57:54Z (GMT). No. of bitstreams: 2 GUIN-DISSERTATION-2019.pdf: 12101441 bytes, checksum: 3c4224d3e7b92270e415798a9995f463 (MD5) LICENSE.txt: 4209 bytes, checksum: b81203a711fa166c28e2eb01d269aef1 (MD5) Previous issue date: 2019-08-23"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/106142"],"dc:language":["en"],"dc:rights":["Copyright 2019 Drishti Guin"],"dc:subject":["Protein binding, in cell, in vitro, dodine, denaturant, protein interactions"],"dc:title":["Protein interactions in vitro and in the cell"],"dc:type":["text"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:45Z"}