Changes in Mammalian Chromatin Structure as a Function of Protein-Poly(ADP-Ribosyl)ation by Endonuclease Digestion

Perez-Lamiguerio, Maria A., Changes in Mammalian Chromatin Structure as a Function of Protein-poly(ADP-ribosyl)ation by Endonuclease Digestion. Master of Science (Biochemistry and Molecular Biology), June 2004. 66 pages, 12 illustrations, Bibliography, 45 titles. Mammalian chromatin was exposed to either Deoxyribonuclease I or Micrococcal Nuclease digestion as a function of time of incubation and enzyme concentration. Endonuclease enzymatic reactions were stopped with EDTA. Samples were run in 1.5% agarose gels and the oligonucleosomal electrophoretic migration patterns compared. Endonuclease experiments were carried out with rat liver chromatin pre-incubated in the presence or absence of 200 μM βNAD+. A solution of 1.0 mM benzamide was used to stop enzymatic modification. The electrophoretic observations demonstrated a faster and increased degradation of chromatin when proteins were poly(ADP-ribosyl)ated prior to digestion. These results support the hypothesis that that the covalent poly(ADP-ribosyl)ation of chromatin proteins, particularly histones, induces a more relaxed structure, rendering chromatin more sensitive to endonuclease digestion.

Author dc:creator

• Perez-Lamigueiro, Maria A.

Contributors dc:contributor

• Alvarez, Rafael
• Das, Hriday K.
• Basu, Alakananda

• Cell Anatomy
• Cell and Developmental Biology
• Cell Biology
• Cellular and Molecular Physiology
• Computational Biology
• Genetics
• Genetics and Genomics
• Genomics
• Life Sciences
• Medical Cell Biology
• Medical Genetics
• Medicine and Health Sciences
• Molecular Genetics
• Other Cell and Developmental Biology
• Other Genetics and Genomics
• Mammalian chromatin structure
• function
• poly(ADP-ribosyl)ation
• endonuclease digestion
• deoxyribonuclease
• micrococcal nuclease digestion
• enzymatic reactions
• EDTA
• rat liver chromatin
• proteins
• histones

Language dc:language

en

Handle dc:identifier.uri

https://hdl.handle.net/20.500.12503/29638

OAI identifier oai:identifier

oai:tdl-ir.tdl.org:20.500.12503/29638