Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"deoxyribonuclease"”.
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Deoxyribonuclease probing on sea urchin embryo chromatin
… embryos using micrococcal nuclease, pancreatic deoxyribonuclease (DNase I) and restriction endonucleases. Micrococcal nuclease and restriction endonuclease digestions of sea urchin gastrula chromatin have been analysed and it is shown that it is not possible to isolate large polynucleosomal …
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Changes in Mammalian Chromatin Structure as a Function of Protein-Poly(ADP-Ribosyl)ation by Endonuclease Digestion
… Mammalian chromatin was exposed to either Deoxyribonuclease I or Micrococcal Nuclease digestion as a function of time of incubation and enzyme concentration. Endonuclease enzymatic reactions were stopped with EDTA. Samples were run in 1.5% agarose gels and the oligonucleosomal …
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Neutrophil extracellular traps in periodontitis
… a survival advantage when challenged with NETs. Deoxyribonuclease (DNase) secretion, capable of degrading NETs, and resistance to killing by histone, a core structural and antimicrobial protein within NETs, were both shown to be relatively common characteristics possessed by pathogens associated …
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A taxonomic study of the genus Campylobacter
… growth, alkaline phosphatase activity, and deoxyribonuclease (DNase) activity proved to be the most useful phenotypic characteristics for identifying these strains at the species, subspecies and biovar levels.
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Assessment of chromatin activity in mouse and human tissues
Pancreatic deoxyribonuclease preferentially digests active genes during all phases of the cell cycle including mitosis. Recently, a DNAse I-directed in ~ nick translation technique has been used to demonstrate differences in the DNAse I sensitivity of euchromatic and heterochromatic regions of …
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Cytochemical studies on Trypanosoma ranarum
… use of the enzymes ribonuclease (RNAase) and deoxyribonuclease (DNAase) to demonstrate the distribution of nucleic acids in the cytoplasmic inclusions and in the nucleus.</p><p>Sen (1930) reported a technique for locating the enzyme urease within a tissue. His method depends on the action of …
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Comparison of the Protein Contents of Bovine Zymogen Granules and of Pancreatic Juice
… chymotrypsinogen B, procarboxypeptidase B, deoxyribonuclease and procarboxypeptidase A have been located in the chromatograms as 13 distinct peaks on the effluent curves. Complete recovery of the protein in pancreatic juice has been attained in this procedure; the recovery of the protein …
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Resolving the Role of POU1F1 in Human Growth Hormone Locus Activation
… locus control region (LCR) composed of five deoxyribonuclease I hypersensitive sites (HSs). The region encompassing HSI and HSII contains the predominant pituitary somatotrope-specific hGH-N activation function of the LCR. This activity was attributed primarily to POU1F1 (Pit-1) elements at …
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A CHARACTERIZATION OF THE BACTERIOPHAGE 41C AND ITS LYTIC CYCLE IN BACILLUS SUBTILIS 168
… in size, morphology, sensitivity of infection to deoxyribonuclease or streptomycin, plaquing efficiency, host range, and incorporation of ('3)H-thymidine into their DNA. Differences were detected in the molecular weights of their structural proteins and in their plaque morphologies. Phage SPP1 …
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ELECTROPHORETIC AND ENZYMATIC IDENTIFICATION OF PROTEINS ASSOCIATED WITH THE DEVELOPMENT OF COMPETENCE IN BACILLUS SUBTILIS
… of nucleases is described. Using purified deoxyribonuclease I (bovine pancreas), as little as 10 pg of nuclease can be detected. Isoelectric focusing (IEF) and non-equilibrium pH gradient electrophoresis (NEPHGE) were compared as first dimensions, in combination with SDS electrophoresis as …
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Biophysical and Biochemical Properties of Salmonella Potsdam Bacteriophage P3/2
… to be 53.0 percent.</p> <p>By means of deoxyribonuclease and snake venom phosphodiesterase enzyme degradation and mononucleotide separation by ion-exchange chromatrography, the following molar ratios for the P3/2<sup>vir </sup>DNA were determined: G-C 54.7-55.1 percent; A-T 44.9-45.3 …
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Acute post streptococcal glomerulonephritis at Red Cross War Memorial Children's Hospital, Cape Town, South Africa - a five-and half-year descriptive review
… children. 90 (94%) children had elevated anti-deoxyribonuclease B antibodies (anti-DNase-B) levels, and 77 (80%) also had elevated anti-streptolysin O titres (ASOT) titres at presentation. Eighty-eight (92%) children received a diuretic agent, 60 (63%) required an anti-hypertensive agent, and …
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Biochemical and physiological changes in bovine sperm during extended incubation under aerobic and anaerobic conditions
… situ acid denaturation. Inclusion of a general deoxyribonuclease inhibitor aurintricarboxylic acid in the diluent dramatically decreased sperm chromatin stability under both aerobic and anaerobic conditions, suggesting that this inhibitor had a damaging effect on sperm DNA. The effects of …
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Novel antimicrobial combinations for treatment against pathogens associated with infected diabetic foot ulcers
Many promising conventional antimicrobial agents have low permeability and restricted biodistribution, their encapsulation into efficient drug delivery carriers is crucial to increasing their bioavailability and retention time. Lipodisq® (LQ) nanoparticles consist of a circular phospholipid bilayer …